[Histonet] Iron Staining of Bone Marrows

[Tarango, Mark]

Is the problem only on the smears?  If it is maybe its got a bunch of
fatty stuff on top the smears and runs off the slide when you stain on
the machine.  

Why automate Prussian blue anyway?  I'm guessing it costs 10x as much
too.


Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Mobile (702) 759-9229
Fax (702) 939-7663
  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Schaundra Walton
Sent: Wednesday, October 10, 2007 9:04 AM
To: Histonet
Subject: [Histonet] Iron Staining of Bone Marrows

We use a Vetana NEXUS special stainer and stain our bone marrow
aspirates and smears with Ventana's iron kit.  One of our pathologists
has complained (repeatedly) that our irons aren't staining like he
expects.  Has anyone else had trouble with Ventana's iron kit?  Is
anyone using a different protocol?  Any advise would be greatly
appreciated.

Schaundra Walton BS HTL(ASCP)
Swedish American Hospital
1401 E. State St. 
Rockford, IL 61104
       
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"EMF <nvcancer.org>" made the following annotations.
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