[Histonet] CRYOSTAT TROUBLESHOOTING

[anitathorn <@t> comcast.net]

That's exactly how I do it too.  The trick is pulling the chuck off of the freeze bar when there is still a liquid center and then placing the tissue in this "puddle".  I then add a little more OCT on top, put the chuck back onto the freeze bar and weigh this down with the heat extractor.  If I don't catch it before it freezes all the way, I simple score the OCT with a scalpel blade to help create a bond with the subsequently added  RT tissue.  
 -------------- Original message ----------------------
From: "Sebree Linda A." <LSebree <@t> uwhealth.org>
> Marcy,
> 
> I was always taught to apply the OCT to a RT chuck, put in the cryostat
> and when you start to see the OCT turn white, i.e. the OCT in the
> grooves is starting to freeze, add the tissue and weight down with the
> cold heat extractor.
> 
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> MARCELLYN STONE
> Sent: Tuesday, October 09, 2007 9:40 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] CRYOSTAT TROUBLESHOOTING
> 
> 
> Hello..
> 
> I am looking for some help.  The O.C.T. embedded tissue is falling of
> the
> chuck when we try to section.  Does anyone have any ideas to stop this?
> We
> have tried new O.C.T., cleaning chucks, advancing slower.  Please help.
> Thanks  Marcy
> 
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