[Histonet] CRYOSTAT TROUBLESHOOTING

[Sebree Linda A.]

Marcy,

I was always taught to apply the OCT to a RT chuck, put in the cryostat
and when you start to see the OCT turn white, i.e. the OCT in the
grooves is starting to freeze, add the tissue and weight down with the
cold heat extractor.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MARCELLYN STONE
Sent: Tuesday, October 09, 2007 9:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CRYOSTAT TROUBLESHOOTING


Hello..

I am looking for some help.  The O.C.T. embedded tissue is falling of
the
chuck when we try to section.  Does anyone have any ideas to stop this?
We
have tried new O.C.T., cleaning chucks, advancing slower.  Please help.
Thanks  Marcy

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