[Histonet] can I leave IHC sections overnight after first staining?

[Mike Pence]

Why would you do all the staining, then stop something that takes less
the 10 more minutes to finish?
Just asking.

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Moran
Elishmereni
Sent: Monday, October 08, 2007 11:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] can I leave IHC sections overnight after first
staining?


Hi Histonetters,

Is it possible to leave sections (that were stained with primary Ab)
overnight, and counterstain the day after? I am using DAB reaction. Can
I leave it dry (or wet, in distilled water) overnight?

Thanks,
Moran

-- 
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish <@t> gmail.com
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