Hi all, I have no experience with IHC but (once again) had a researcher ask me if placing tissue in sucrose after 10% NBF is helpful in antigen staining. Thanks, Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax)