[Histonet] Mammaglobin A & GCDFP-15

Dana Settembre settembr <@t> umdnj.edu
Tue Oct 2 10:57:18 CDT 2007


Hello Brett,
I cannot help you with Mam A but I do have a supplier for GCDFP-15.
I use Signet Pathlogy Systems in Dedham, Mass.
1-800-223-0796
I use their GCDFP-15 (Brst-2) clone D6 on human tissue.  It is made in
mouse.
We rarely use it so I buy their prediluted 6ml vial.   I dilute that
1:1.
I use Dako's Ready to Use Prot. K for 5 minutes @ RT.
Hope this helps.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Connolly, Brett M" <brett_connolly <@t> merck.com> 09/28/07 4:00 PM
>>>
Hi all,
Looking for suppliers of these antibodies for FFPE tissues. Also,
necessary pretreatments/dilutions used.

Thanks,
Brett

Brett M. Connolly, Ph.D.
Research Fellow
MRL, Imaging Research
Merck & Co., Inc.
WP-44K
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-993-6803
e-mail. brett_connolly <@t> merck.com 


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