[Histonet] Wrinkles and folds in paraffin sections

Fri Nov 30 12:08:04 CST 2007

Dear Rebecca,
To my understanding, wrinkles do usually appear when the speed of cutting is
not correct with hesitations. The cutting has to be smooth, homogeneous
speed without stops or hesitations. Too cold paraffin frequently increases
this but it is usually mainly due to handling.
Motorized microtome can also help by controlling speed but it costs !
Good luck
Pierre

Rebecca Barnhart <RBARNHART <@t> summithealth.org> wrote:
  Recently we started monitoring slide quality in the histology department.
We have noticed a trend of folds/wrinkles. I have changed paraffins from
Surgipath Blue Ribbon to Fisher's Paraplast X-tra and it does not seem to
have made a difference. I have tired using Halt which didn't make a
difference either. Most of our specimens are GI biopsies, curettings, skins
and breast biopsies. Any other suggestions? Thanks for the help.

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Objet : Histonet Digest, Vol 48, Issue 42

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Today's Topics:

   1. Re: Slide quality (Rene J Buesa)
   2. RE: SPAM-LOW:  [Histonet] Slide quality (Douglas D Deltour)
   3. remove from list (Stacey Burton)
   4. Decal Solutions and Her2 Fish (Disher Lori)
   5. sudan black stain for fecal fat (Irene Cochran)
   6. CDX2 and HCA (hepatocellular carcinoma) (Piche-Grocki, Jessica)
   7. Re: Decal Solutions and Her2 Fish (Rene J Buesa)
   8. Re: sudan black stain for fecal fat (Rene J Buesa)
   9. Amyloid (Lorraine Cornett)

----------------------------------------------------------------------

Message: 1
Date: Fri, 30 Nov 2007 08:04:46 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Slide quality
To: Rebecca Barnhart <RBARNHART <@t> summithealth.org>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <479329.35997.qm <@t> web61223.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Usually wrinkles are the consequence of poor infiltration or poor microtomy
practices more than on the paraffin's quality.
  Check if you have changed anything on your processing schedule or in some
of the techs' sectioning habits. Do wrinkles appear on sections from
everybody? A section QA program has to be able to identify who prepared the
slides.
  Reni J.

Rebecca Barnhart <RBARNHART <@t> summithealth.org> wrote:
  Recently we started monitoring slide quality in the histology department.
We have noticed a trend of folds/wrinkles. I have changed paraffins from
Surgipath Blue Ribbon to Fisher's Paraplast X-tra and it does not seem to
have made a difference. I have tired using Halt which didn't make a
difference either. Most of our specimens are GI biopsies, curettings, skins
and breast biopsies. Any other suggestions? Thanks for the help.

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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Message: 2
Date: Fri, 30 Nov 2007 11:18:21 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: SPAM-LOW:  [Histonet] Slide quality
To: "'Rebecca Barnhart'" <RBARNHART <@t> summithealth.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1196445600.11087.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="US-ASCII"

Rebecca,

I would defiantly check to see if the wrinkles are generated by one
particular person. My techs initial the slides that they cut. This is the
easiest way to see if things of this nature are a trend. 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
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any dissemination, distribution, or copying of this communication is
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Barnhart
Sent: Friday, November 30, 2007 10:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Slide quality

Recently we started monitoring slide quality in the histology department.
We have noticed a trend of folds/wrinkles.  I have changed paraffins from
Surgipath Blue Ribbon to Fisher's Paraplast X-tra and it does not seem to
have made a difference.  I have tired using Halt which didn't make a
difference either.  Most of our specimens are GI biopsies, curettings, skins
and breast biopsies.  Any other suggestions?  Thanks for the help.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 3
Date: Fri, 30 Nov 2007 08:31:25 -0800 (PST)
From: Stacey Burton <staceylburton <@t> yahoo.com>
Subject: [Histonet] remove from list
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <630300.59097.qm <@t> web30007.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Please remove me from the mailing list.

Thank you,
Stacey Burton

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Message: 4
Date: Fri, 30 Nov 2007 11:31:06 -0500
From: "Disher Lori" <Lori.Disher <@t> HCAhealthcare.com>
Subject: [Histonet] Decal Solutions and Her2 Fish
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<095327C7CDBDF64B9E9728A54799091E33A593 <@t> ORLEV03.hca.corpad.net>
Content-Type: text/plain;	charset="iso-8859-1"

Hello,
Can anyone recomend a decal solution to use that will not destroy the cells
for HER 2 Fish?  If so, do you have a policy?

Lori Disher
Fawcett Memorial Hosp
21298 Olean Blvd
Port Charlotte,  FL  33952
941-627-6128

------------------------------

Message: 5
Date: Fri, 30 Nov 2007 09:25:31 -0800 (PST)
From: Irene Cochran <ifc1028 <@t> yahoo.com>
Subject: [Histonet] sudan black stain for fecal fat
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <746750.38134.qm <@t> web45608.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Our pathologist is looking for a procedure on staining fecal fat with sudan
black any suggestions?  thanks

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Message: 6
Date: Fri, 30 Nov 2007 12:33:53 -0500
From: "Piche-Grocki, Jessica" <jpiche-grocki <@t> wtbyhosp.org>
Subject: [Histonet] CDX2 and HCA (hepatocellular carcinoma)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<0A57D6AEAE4CBA4A984D27257160A72D01069D0B <@t> win03exchange01.wtbyhosp.org>

Content-Type: text/plain;	charset="iso-8859-1"

Hello,

I am having a hard time finding information for our pathologists on CDX2 and
HCA antibodies. If anyone has any information they can share that would be
great. I am especially having a hard time with the HCA(hepatocellular
carcinoma antigen). 

Thanks and have a great weekend!! 

Jessica Piche-Grocki, HT(ASCP)

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Message: 7
Date: Fri, 30 Nov 2007 09:39:42 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Decal Solutions and Her2 Fish
To: Disher Lori <Lori.Disher <@t> HCAhealthcare.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <916852.26259.qm <@t> web61213.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We used RDO and EDTA for more delicate specimens (and BM) and after that we
regularly did IHC on those sections. I don't think that Her2 neu FISH would
behave any differently, although I never did that (FISH always on breast
tumors not requiring decal).
  Reni J.

Disher Lori <Lori.Disher <@t> HCAhealthcare.com> wrote:
  Hello,
Can anyone recomend a decal solution to use that will not destroy the cells
for HER 2 Fish? If so, do you have a policy?

Lori Disher
Fawcett Memorial Hosp
21298 Olean Blvd
Port Charlotte, FL 33952
941-627-6128
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Message: 8
Date: Fri, 30 Nov 2007 09:41:57 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] sudan black stain for fecal fat
To: Irene Cochran <ifc1028 <@t> yahoo.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <57495.58444.qm <@t> web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

And don't you use Oil Red O? You have not processed the feces and could use
the ORO
  Reni J.

Irene Cochran <ifc1028 <@t> yahoo.com> wrote:
  Our pathologist is looking for a procedure on staining fecal fat with
sudan black any suggestions? thanks

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Message: 9
Date: Fri, 30 Nov 2007 12:49:26 -0500
From: Lorraine Cornett <cornettl <@t> hotmail.com>
Subject: [Histonet] Amyloid
To: Histonet Listserve <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU106-W49C36D0B85A11FFD5957DBD6710 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Thanks to everyone on the histonet, I have a couple of control blocks on the
way!

Have a great weekend!Lorraine Cornett, HT (ASCP)Highlands PathologyBlue
Ridge Division, Kingsport, TN423 224-5793 fax 423 224-5349
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