[Histonet] Immunofluorescence

Neehar Bhatia nbhatia <@t> dermatology.wisc.edu
Thu Nov 29 12:28:58 CST 2007

Generator Microsoft Word 11 (filtered medium) Hi Everybody,
I plan to do immunofluorescence in mouse tissue. I saw a protocol where they have fixed the tissue in 95/1% ethanol/acetic acid and the made paraffin blocks. Why is this fixation protocol used instead of formalin? Does it help in eliminating autofluorescence?
Thanks for your help

Neehar Bhatia, Ph.D
Assistant Scientist,
Department of Dermatology,
University of Wisconsin,
445 Henry Mall, Rm 302,
Madison WI 53706
Ph 608.262.3239
Fax 608.263.2919

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