[Histonet] substance P receptor

[Angelina Fong]

Hi Neil,

I have had some lovely substance P receptor (or Neurokinin 1 receptor) 
labelling in rat brains using a rabbit polyclonal antibody from Novus 
Biological/  I don't have the catalogue number on hand, but let me know 
if you want it.  I have never processed tissue for NK1R labeling with 
picric acid, but have used just straight 4% PFA transcardial perfusion 
after saline and labelling/staining looks great, both in fluorescence or 
DAB.  I have used a guinea pig anti-NK1R antibody that didn't work quite 
as well as the one from Novus.  Let me know if you would like more 
specifics. 

Good luck
Angelina


> Does anybody have experience staining rat brain for the substance P receptor? Which company would you recommend? I have also read that many researchers fix tissue with paraformaldehyde and picric acid? Unfortunately, when we designed our experiments we did not anticipate that we would have to stain our tissue with this antibody and I was wondering how important is it to fix tissue with a paraformaldehyde/picric acid solution. Our rats were perfused with physiological saline followed by 4% paraformaldehyde. Brains were removed and postfixed for 72 hrs in the same solution, before being placed in 0.1% sodium azide/PB solution prior to sectioning on a Vibratome at 50 micron. 
>
> Thanks for the help,
>
> Neil
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-- 
~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o

Angelina Y. Fong, Ph.D.
Department of Zoology
Biological Sciences Building
6270 University Boulevard
University of British Columbia
Vancouver, BC, V6T 1Z4
Canada 

Ph:  (604) 822-5990
Fax: (604) 822-2416
Email: fong <@t> zoology.ubc.ca