[Histonet] RE: need cd19 help in mouse spleens and a job

[Milne, Katy]

Hi Nick, I wasn't very successful at finding a CD19 Ab for mouse FFPE but I did find a PAX5 Ab that worked for detecting B cells, it came from Lab Vision (RB-9406), it should give nuclear staining in B cells but not in plasma cells though.  You could potentially try CD79a for that as I think that comes up strong in plasma cells but again getting a good Ab for mouse FFPE for that is tricky.

Katy Milne
Deeley Research Centre

Message: 5
Date: Mon, 26 Nov 2007 11:48:41 -0500
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] need cd19 help in mouse spleens and a job
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <8F3E1865E343C943BB38506D56FF015101335516 <@t> MD1EV002.medimmune.com>
Content-Type: text/plain;       charset="us-ascii"

I need help finding a source and even a decent procedure that works on
cd19 on FFPE mouse spleens.  I have a decent procedure for cd3 and cd45,
but the path wants cd19, so I figured I would throw it to the experts
first.  Thanks in advance.  Also Medimmune is looking for a histotech
here in Rockville Md.  Someone with GLP, frozen and IHC experience.



Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)



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End of Histonet Digest, Vol 48, Issue 36
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