[Histonet] Update: quantifying only the surface-expressed levels of a receptor in sections

[Kevin Clayton]

Sorry to mail again but I just noticed that as the brains were
cryoprotected in glycerol/ethylene glycol/PBS they've already been
permeabilized (right?).

Kevin


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Hi all

As the subject says, I want to quantify only the surface levels of
glutamate receptors in mouse brain vibratome sections. I want to see
if there are more/less receptors from mice that have received a
certain treatment. Now that I have the antibodies working well I
realise that it may not even be possible to specifically look at the
level of the receptors in the cell membrane (excluding those in
intracellular vesicles, for example). Is it possible? I don't
permeabilize of course but sectioning itself must damage the integrity
of the cells. Or can that be overcome by only taking confocal slices
from further in the tissue?

Thanks

Kevin