[Histonet] frozens from lung-tissue

Kemlo Rogerson Kemlo.Rogerson <@t> waht.swest.nhs.uk
Fri Nov 23 09:23:49 CST 2007

My original question was, how to get the best frozen lung-slides without
the use of liquid nitrogen or isopentan. - just with the cryocut, chucks
and OCT. The pathologist complains about "overfrozen" edges and a bad
overall morphology. I will ask him for more descriptionary details or
get a picture. Thank you for your hints.

Gudrun Lang

But that's the thing I'm struggling with. Liquid nitrogen with or
without 'additives' is the best way of reducing ice artefact, so by not
using it I expect you to get worse results. If you want 'better'
morphology then post fix. 

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
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