AW: [Histonet] frozens from lung-tissue

[soofia siddiqui]

I get the best quality sections when I snap freeze the tissue ( very small not lungs) in dry ice and isopropenal bath. Add a little isopropenal into dry ice to make the bath , use small plastic beem/ beam ( Energy Beam Sciences) capsule, fill with liquid OCT, place the tissue in the capsule with liquid OCT, put hanging tag( some thing to hold into bath or information), cover the capsule  and dip the capsule for few seconds. You can see the freezing of OCT, just pull it out from the bath. We in our lab usually leave the frozen tissue  in -80 Celsius degrees for over night and then cut next day.
  Soofia
  UWHC Madison WI

       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.