[Histonet] Histogel Question

Dana Settembre settembr <@t> umdnj.edu
Wed Nov 21 10:37:44 CST 2007


Abbie,
I have used Histogel to embed very small human eye biopsies.
What do you mean, "that obviously is not placed in a tube?"?
We pick up the small specimen carefully with forceps and place
the tissue at the very bottom of the gel and when it goes into 
a cassette, it is placed facing down.  We make sure that the
embedding histotech understands exactly where it is for
processing and embedding.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> "Abigail M. Butler" <am3309 <@t> uga.edu> 11/21/07 10:13 AM >>>
I completely understand how to use histogel to embed free floating
cells. But how would I embed a piece of tissue that obviously is not
placed in a tube?
I am considering purchasing Histogel for the first time and have many
ideas of how I could use it, just not sure the proper steps to take to
embed let's say two mice eyes.
Thanks
Abbie Butler, HT (ASCP), QIHC
University of Georgia
College of Veterinary Medicine

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