[Histonet] Formaline-free fixative
Pierre CHAUMAT
pierre.chaumat <@t> alphelys.com
Tue Nov 20 15:38:58 CST 2007
Hello all,
We have developed a new fixative named RCL2 that provides a molecular
quality close to frozen sections but with the morphology obtained with
formaline.
IHC requires much less retrieval, naturally. We have demonstrated stability
of samples over 7 years.
RCL2 also replaces advantageously deep freezing as RCL2 fixed tissues can be
stored simply at -20°C thus generating large costs savings and simplicity
for biobanking.
Penetration speed of RCL2 is similar to formaline but you can leave your
tissues into RCL2 without any damages on morphology nor signals.
And to keep it for the end...RCL2 is absolutely non toxic and even better :
drinkable for a "strong" man. The content ? A patented complex carbohydrate,
acetic acid at 6% and ethanol at 65% (thus the "strong" man).
We have hospitals and private labs starting to use it in routine.
I want also to add that the French Cancer Research Institute is starting to
test it for Her2neu after having given up on many other proposed fixatives
with glyoxal based fixatives amongst them because of over fixation.
Anyone here interested in testing it ? Motivated to change to a new fixative
for real benefits ?
Kind regards
Pierre
-----Message d'origine-----
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[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] De la part de
histonet-request <@t> lists.utsouthwestern.edu
Envoyé : mardi 20 novembre 2007 19:14
À : histonet <@t> lists.utsouthwestern.edu
Objet : Histonet Digest, Vol 48, Issue 28
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Today's Topics:
1. atlanta jobs (Cutter2754 <@t> aol.com)
2. RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
(Douglas D Deltour)
3. Metallic sheen on hematoxylin (Johnson, Mindy)
4. re:B-5 solution (ATOJSU <@t> aol.com)
5. RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
(Bernice Frederick)
6. Re: Metallic sheen on hematoxylin (Rene J Buesa)
7. Cindy Higgerson is out of the office. (chiggerson <@t> memhosp.com)
8. RE: SPAM-LOW: Re: [Histonet] no subject (Ingles Claire)
9. IHC for MMP2, MMP9, TIMP1 and TIMP2? (AGrobe2555 <@t> aol.com)
10. RE: re:B-5 solution (Lee & Peggy Wenk)
11. (no subject) (kamal prasad)
12. RE: QIHC (Goodpaster, Tracy A)
13. Prognostic factors in colorectal cancers (K M)
14. Re: forceps and bunsen burners (Joe Nocito)
15. New RA Lamb Cassette Printer UK Only (Victor Tobias)
16. Histologist Job in ATL GA (Tara Ollis)
17. Matrigel as culture medium - try agarose embedding
(MVaughan4 <@t> ucok.edu)
----------------------------------------------------------------------
Message: 1
Date: Mon, 19 Nov 2007 13:12:03 EST
From: Cutter2754 <@t> aol.com
Subject: [Histonet] atlanta jobs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <d5f.17a0891f.34732bf3 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hi all,
I'm moving to the Atlanta area in a few months and would like to know about
the employment situation. I found several jobs available but would also
like to hear about people's experiences - the good and the bad!
Thanks,
Kathy
************************************** See what's new at http://www.aol.com
------------------------------
Message: 2
Date: Mon, 19 Nov 2007 13:13:33 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
To: "'Sate Hamza'" <dermpathsy <@t> gmail.com>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1195581600.4819.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Invitrogen-Zymed has a pre-dilute that can be used in a prep-kit on the XT.
This is the same one that I am currently troubleshooting.
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sate Hamza
Sent: Monday, November 19, 2007 12:23 PM
To: Douglas D Deltour; Histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
Thanks to all who have responded to my message.
I asked my colleague and she said that she has not ordered any antibody
yet. She said she wanted to see if she can get a protocol first before she
orders the antibody.
She was wondering what the source of your antibody was ..
Thanks again ..
Sate
On 11/19/07, Douglas D Deltour <doug <@t> ppspath.com> wrote:
>
> We just started using the MITF-1 antibody on the XT. I got it validated
> and
> we were running it for about 2 months and then all of a sudden it stopped
> working, but that is another story. Which company did your colleague get
> the
> antibody from? I used the same protocol that I used for the MART-1 for
> validation. This was working great for the MITF until.....
>
> [---]
>
>
>
>
--
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada
_______________________________________________
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------------------------------
Message: 3
Date: Mon, 19 Nov 2007 10:14:31 -0800
From: "Johnson, Mindy" <m5johnso <@t> ucsd.edu>
Subject: [Histonet] Metallic sheen on hematoxylin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D458FDD1DF682843887073F69CE67F54144D65B018 <@t> meded-staexchan.AD.UCSD.EDU>
Content-Type: text/plain; charset="us-ascii"
Ok, I was trying to find where I found this information, but I can't find
it, so.... The metallic sheen I get on top of my hematoxylin, I though was
very concentrated (this is the information I was talking about). Is this
true and if so, what can I mix it with to extend the life of it? Thanks!
Mindy
Medical Teaching Labs, UCSD
------------------------------
Message: 4
Date: Mon, 19 Nov 2007 14:07:28 EST
From: ATOJSU <@t> aol.com
Subject: [Histonet] re:B-5 solution
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <cca.1ffd3565.347338f0 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
We have 1 Pathologist who insists on using B-5 solution for nuclear detail
on small biopsies. How many labs are still using B 5 or what is you
suggestion other than Zinc Formalin for fixation. Thanks.
************************************** See what's new at http://www.aol.com
------------------------------
Message: 5
Date: Mon, 19 Nov 2007 13:20:53 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
To: "'Douglas D Deltour'" <doug <@t> ppspath.com>, "'Sate Hamza'"
<dermpathsy <@t> gmail.com>, <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c82ae1$50b449e0$d00f7ca5 <@t> lurie.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
LabVision also has a pre-dilute for clone D5,which is what we use undiluted.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Douglas D
Deltour
Sent: Monday, November 19, 2007 12:14 PM
To: 'Sate Hamza'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
Invitrogen-Zymed has a pre-dilute that can be used in a prep-kit on the XT.
This is the same one that I am currently troubleshooting.
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sate Hamza
Sent: Monday, November 19, 2007 12:23 PM
To: Douglas D Deltour; Histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer
Thanks to all who have responded to my message.
I asked my colleague and she said that she has not ordered any antibody
yet. She said she wanted to see if she can get a protocol first before she
orders the antibody.
She was wondering what the source of your antibody was ..
Thanks again ..
Sate
On 11/19/07, Douglas D Deltour <doug <@t> ppspath.com> wrote:
>
> We just started using the MITF-1 antibody on the XT. I got it validated
> and
> we were running it for about 2 months and then all of a sudden it stopped
> working, but that is another story. Which company did your colleague get
> the
> antibody from? I used the same protocol that I used for the MART-1 for
> validation. This was working great for the MITF until.....
>
> [---]
>
>
>
>
--
Sate Hamza, MD, FRCPC
Dermatopathologist
Winnipeg, Canada
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 19 Nov 2007 12:24:36 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Metallic sheen on hematoxylin
To: "Johnson, Mindy" <m5johnso <@t> ucsd.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <414329.50634.qm <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Metallic sheen on the surface of the hematoxylin is due to a surface
oxydation. The best way of dealing with it is to take a kin-wip and skim the
surface with it. Another solution (more cumbersome) is to filter the
hematoxylin.
Do not mix anything with the hematoxylin because then you will end with a
different formula.
This is a surface chemical product that can be eliminated mechanically.
Reni J.
"Johnson, Mindy" <m5johnso <@t> ucsd.edu> wrote:
Ok, I was trying to find where I found this information, but I can't find
it, so.... The metallic sheen I get on top of my hematoxylin, I though was
very concentrated (this is the information I was talking about). Is this
true and if so, what can I mix it with to extend the life of it? Thanks!
Mindy
Medical Teaching Labs, UCSD
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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------------------------------
Message: 7
Date: Mon, 19 Nov 2007 15:02:25 -0600
From: chiggerson <@t> memhosp.com
Subject: [Histonet] Cindy Higgerson is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF41DEE52D.762F3C8A-ON86257398.0073943A-86257398.0073943A <@t> pmmc.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting Mon 11/19/2007 and will not return
until Tue 11/27/2007.
I will respond to your message when I return.
------------------------------
Message: 8
Date: Mon, 19 Nov 2007 15:15:02 -0600
From: "Ingles Claire" <CIngles <@t> uwhealth.org>
Subject: RE: SPAM-LOW: Re: [Histonet] no subject
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<08A0A863637F1349BBFD83A96B27A50A1200A2 <@t> uwhis-xchng3.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
I am! I'll even boycott Friday too!
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Douglas D
Deltour
Sent: Mon 11/19/2007 9:01 AM
To: 'Emily Sours'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: SPAM-LOW: Re: [Histonet] no subject
I honor of this thread I declare Thursday November 22nd National Histology
Strike Day! We will not report to work on this day to show our
dissatisfaction. Who is with me?
------------------------------
Message: 9
Date: Mon, 19 Nov 2007 18:04:33 EST
From: AGrobe2555 <@t> aol.com
Subject: [Histonet] IHC for MMP2, MMP9, TIMP1 and TIMP2?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <bd0.1f302efa.34737081 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello all,
I have a panel of tissues (Large and small intestine, liver, spleen,
kidney,
heart, esophagus, lung) as well as normal artery and vein tissue (all are
from sheep) that we are using to test/optimize IHC for MMP2, MMP9, TIMP1
and
TIMP2. We have stained for MMP9 so far, and see some staining in cells
(appear
to be macrophages) located in the small and large intestine, and in the
adventitial/ablumenal tissue of the vein. We will be doing the other
stains in
the days to come.
My difficulty is that I am in unknown territory with these antibodies. Has
anyone of you stained for these before? What should I be looking for as a
positive signal in these tissues? Can anyone point me to some reference
images? In the interim, I will continue to look for information as the
product
sheets are not really providing information as to appropriate + control
tissues.
Thank you,
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
************************************** See what's new at http://www.aol.com
------------------------------
Message: 10
Date: Mon, 19 Nov 2007 19:33:31 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] re:B-5 solution
To: <ATOJSU <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201c82b0c$faad1c30$0202a8c0 <@t> HPPav2>
Content-Type: text/plain; charset="us-ascii"
Why not zinc formalin? Usually, if people have tried zinc formalin and
didn't like it, it's because they didn't leave the tissue in the zinc
formalin long enough. Usually they have left it for the same amount of time
as the B5, and as a result the nuclear detail is lousy.
The secret is that zinc salts binds a little slower than mercuric chloride.
So take your B5 time, and multiply it by 1.5, and that will be a good
starting point for zinc formalin. In other words, if the biopsies were fixed
in B5 in 2 hours, then 2 x 1.5 = 3 hours would be a good guess at how long
to fix in zinc formalin.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ATOJSU <@t> aol.com
Sent: Monday, November 19, 2007 2:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re:B-5 solution
We have 1 Pathologist who insists on using B-5 solution for nuclear detail
on small biopsies. How many labs are still using B 5 or what is you
suggestion other than Zinc Formalin for fixation. Thanks.
************************************** See what's new at http://www.aol.com
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 20 Nov 2007 01:28:23 +0000 (GMT)
From: kamal prasad <peddinti_2002us <@t> yahoo.co.in>
Subject: [Histonet] (no subject)
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <728317.58538.qm <@t> web8705.mail.in.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Yes , 10% formic acid is best decalcifier it will keep cell morphology
better.
Formic acod need to mix it with 10%NBF .
---------------------------------
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here.
------------------------------
Message: 12
Date: Mon, 19 Nov 2007 17:37:40 -0800
From: "Goodpaster, Tracy A" <tgoodpas <@t> fhcrc.org>
Subject: RE: [Histonet] QIHC
To: "Tarango, Mark" <mtarango <@t> nvcancer.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BD118D3F85CFB64CA2E14F6D53F76FA81F37C2 <@t> ISIS.fhcrc.org>
Content-Type: text/plain; charset="us-ascii"
I am also interested in this information. I don't know how specific it
will be, especially over clinical lab regulations. Any additional
information would be helpful!
Thanks!
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tarango,
Mark
Sent: Sunday, November 11, 2007 3:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] QIHC
To anyone who has taken the QIHC exam, what kinds of questions does it
ask? I've got a few books from the reading list, but I have no idea
where to focus my attention. It is more about staining techniques or
immunophenotyping? Quality control stuff? What's on the test that you
might not expect? Is it a difficult exam?
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
"EMF <nvcancer.org>" made the following annotations.
------------------------------------------------------------------------
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------------------------------
Message: 13
Date: Tue, 20 Nov 2007 11:28:27 +0200
From: "K M" <ombadda3 <@t> gmail.com>
Subject: [Histonet] Prognostic factors in colorectal cancers
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<ac7603c00711200128q61365c0bu45d758ce427bcd05 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Dear colleagues
I am doing study on IHC in colorectal cancers in human tissues and correlate
them with prognosis .I used an antibody for Claudin-1(a tight junction
protein found in cell membrane) as a prognostic factor.Ineed another
prognostic factor for colorectal cancer for that I am enquiring if any one
know other strong prognostic factor used in colorectal cancers?
Dr.K.M.Omer
------------------------------
Message: 14
Date: Tue, 20 Nov 2007 06:08:56 -0600
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] forceps and bunsen burners
To: "Cheryl R. Kerry" <tkngflght <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <015d01c82b6e$207b2f40$0302a8c0 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
the good ole days. The lab I'm at now used to used micro loop flamers. It
was like playing the game "operation", if you touched the sides, sparks
shoot up. It does cause a nice light show with the lights off. My other
place of employment, we used alcohol burners, others used gauze.
Personally, I miss the days of having a cigarette hanging from my mouth
while cutting and coverslipping. I admit, I'm much healthier today, but miss
the good ole days.
JTT
----- Original Message -----
From: "Cheryl R. Kerry" <tkngflght <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, November 18, 2007 10:21 AM
Subject: [Histonet] forceps and bunsen burners
>I really miss Bunsen burners! Now we use a lot of kimwipes.
>
> Kemlo--I recall the days of wiping your embedding center with a xylene
> soaked rag...me with a cuppa joe on my 'tome and others with cigarettes
> hanging out the sides of their mouths...and yes, the occasional lab fires
> that resulted!! And I really miss REAL light green SF yellowish!
>
> Cheryl
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kemlo
> Sent: Sunday, November 18, 2007 4:22 AM
> To: 'sheila adey'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] (no subject)
>
> In the good old days of Bunsen burners we used to fry the end of the
> forceps; nothing survived that and if it did it was readily identifiable.
> Alas the Bunsen burner has been consigned to the politically incorrect as
> the 'Scientists' of today would incinerate themselves whilst the
> 'Technicians' of yesterday didn't (well not often).
>
> The sad demise of mercury, lead, Bunsen burners, formalin, anything too
> hot,
> too cold, too explosive, too poisonous, etc. Having your tea in the Lab
> next
> to the specimens and processing TB specimens 'on the bench'.
>
> Would Histology have the techniques and stains it now has if harmful
> chemicals had not been experimented with? Will anything new be discovered
> by
> the HistoTech if all that we can use is 'safe' chemicals and procedures? I
> don't see the kids fiddling with things like we use to, no explosions, no
> fires and no injuries. Am I just an old reactionary waiting to be put out
> to
> pasture and ruminate on what was?
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sheila
> adey
> Sent: 17 November 2007 20:41
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> Hi Netters,
>
> We are trying to minimize possible embedding contaminations. What are
> other
> people doing to prevent contamination due to forceps etc.
>
> Thanks in advanceSheila Adey HT MLTPort Huron HospitalMichigan
> _________________________________________________________________
> Are you ready for Windows Live Messenger Beta 8.5 ? Get the latest for
> free
> today!
>
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> _____________________________
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>
>
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
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------------------------------
Message: 15
Date: Tue, 20 Nov 2007 08:08:36 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: [Histonet] New RA Lamb Cassette Printer UK Only
To: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <47430684.4000608 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Has anyone purchased or seen this new cassette labeler? We are still
waiting for Thermo to release the US version. I have heard it will print
cassettes with bar codes in 2.5 seconds, which is remarkable.
Victor
--
Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Message: 16
Date: 20 Nov 2007 11:48:11 -0500
From: Tara Ollis <tara.ollis <@t> teamstaffrx.com>
Subject: [Histonet] Histologist Job in ATL GA
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <18512542.1195577290900.JavaMail.cfservice <@t> webserver29>
Content-Type: text/plain; charset="utf-8"
Hello-
I have a Histologist position available in a dermatologist office in the
greater Atlanta area.
They need someone with MOHS experience and prefer HTL Certification.
Feel free to email me your resume or call me for more details.
Thanks!
Tara Ollis |
TeamStaff Rx, Inc.
Office:
1.877.523.9897 ext.5511
| www.teamstaffrx.com
------------------------------
Message: 17
Date: Tue, 20 Nov 2007 11:54:45 -0600
From: MVaughan4 <@t> ucok.edu
Subject: [Histonet] Matrigel as culture medium - try agarose embedding
To: histonet <@t> lists.utsouthwestern.edu
Cc: TJJ <@t> Stowers-Institute.org
Message-ID:
<OF92BE171F.65F21E12-ON86257399.0061F35A-86257399.0062A2A2 <@t> ucok.edu>
Content-Type: text/plain; charset="US-ASCII"
Teri,
Can you embed or surround the matrigel mixture in a low-melting agarose
first, then postfix the agarose/matrigel mixture in formalin, followed by
paraffin embedding? I have used it with collagen matrices.
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, NSF Sure-Step program
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm
Message: 8
Date: Fri, 16 Nov 2007 14:42:54 -0600
From: "Keller, Charles" <kellerc2 <@t> uthscsa.edu>
Subject: RE: [Histonet] Matrigel as culture medium
To: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A1827A123364E949BDCB52A739347A1D05175BAB <@t> addax.win.uthscsa.edu>
Content-Type: text/plain; charset="us-ascii"
Dear Teri, a possible solution is to use Extracel (from Glycosan Inc in
Utah) instead of Matrigel. I've fixed Extracel in 10% formalin and
sectioned in in paraffin. It has the advantage of being synthetic
(Matrigel has viral contamination from time to time). Sincerely,
Charles
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson,
Teri
Sent: Friday, November 16, 2007 9:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Matrigel as culture medium
One of our researchers is using matrigel as a culture medium on
chambered slides. We tried paraffin embedding them, and found the
Matrigel wasn't really solid enough to hold the cellular structure in
place. We found it particularly difficult to keep some sort of
consistency scraping it off (it had the consistency of warm jelly).
I'd love to hear some ideas on how we can accomplish sample processing
without centrifugation. Ideally it would be good to encase the cells in
a matrix that would solidfy enough to embed as a cell block, keeping the
cellular structure intact.
Thanks for your help!
Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110
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