[Histonet] re:B-5 solution

Lee & Peggy Wenk lpwenk <@t> sbcglobal.net
Mon Nov 19 18:33:31 CST 2007

Why not zinc formalin? Usually, if people have tried zinc formalin and
didn't like it, it's because they didn't leave the tissue in the zinc
formalin long enough. Usually they have left it for the same amount of time
as the B5, and as a result the nuclear detail is lousy. 

The secret is that zinc salts binds a little slower than mercuric chloride.
So take your B5 time, and multiply it by 1.5, and that will be a good
starting point for zinc formalin. In other words, if the biopsies were fixed
in B5 in 2 hours, then 2 x 1.5 = 3 hours would be a good guess at how long
to fix in zinc formalin.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ATOJSU <@t> aol.com
Sent: Monday, November 19, 2007 2:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] re:B-5 solution

We have 1 Pathologist who insists on using B-5 solution for nuclear   detail

on small biopsies.  How many labs are still using B 5 or what is you
suggestion other than Zinc Formalin  for fixation.  Thanks.

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