SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer

Bernice Frederick b-frederick <@t>
Mon Nov 19 13:20:53 CST 2007

LabVision also has a pre-dilute for clone D5,which is what we use undiluted.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Douglas D
Sent: Monday, November 19, 2007 12:14 PM
To: 'Sate Hamza'; Histonet <@t>
Subject: RE: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer

Invitrogen-Zymed has a pre-dilute that can be used in a prep-kit on the XT.
This is the same one that I am currently troubleshooting. 

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> 
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Sate Hamza
Sent: Monday, November 19, 2007 12:23 PM
To: Douglas D Deltour; Histonet <@t>
Subject: SPAM-LOW: Re: [Histonet] MiTF on a Ventana Immunostainer

Thanks to all who have responded to my message.

I asked my colleague and she said that she has not ordered any antibody
yet.  She said she wanted to see if she can get a protocol first before she
orders the antibody.
She was wondering what the source of your antibody was ..

Thanks again ..


On 11/19/07, Douglas D Deltour <doug <@t>> wrote:
> We just started using the MITF-1 antibody on the XT. I got it validated
> and
> we were running it for about 2 months and then all of a sudden it stopped
> working, but that is another story. Which company did your colleague get
> the
> antibody from? I used the same protocol that I used for the MART-1 for
> validation. This was working great for the MITF until.....
> [---]

Sate Hamza, MD, FRCPC
Winnipeg, Canada
Histonet mailing list
Histonet <@t>

Histonet mailing list
Histonet <@t>

More information about the Histonet mailing list