[Histonet] RE: Histonet Digest, Vol 48, Issue 17
Orr, Rebecca
ROrr <@t> enh.org
Mon Nov 19 11:24:11 CST 2007
Hi Leslie,
Our lab is running the Auto TEC from Sakura and it's working wonderfully.
Let me know if you have any questions, we'll be glad to help.
Becky
Becky Orr CLA,HT(ASCP)QIHC
Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2771
Message: 13
Date: Tue, 13 Nov 2007 11:01:02 -0500
From: "Lesley Bechtold" <lesley.bechtold <@t> jax.org>
Subject: [Histonet] automated paraffin embedding systems
To: "Histology Network" <histonet <@t> Pathology.swmed.edu>
Message-ID: <20071113110102345.00000002152 <@t> spikey>
Content-Type: text/plain; charset=us-ascii
Hi,
I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, November 13, 2007 10:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 48, Issue 17
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Today's Topics:
1. Getting a Tissue Processor (Jennifer Sipes)
2. non pathologist grossing vs processing continued
(Vancleave, Vince)
3. RE: non pathologist grossing vs processing continued
(Charles.Embrey)
4. Susie Hargrove is out of the office. (SHargrove <@t> urhcs.org)
5. RE: deplasticizing PMMA sections for staining (Michelle McDonald)
6. MyoD1 (San Tin)
7. RE: TGIF (Farish, Craig)
8. Claudin-1 as a prognostic factor in colorectal cancer (K M)
9. staining of negative controls with IHC (MaryAnn Dixon)
10. Re: staining of negative controls with IHC (Rene J Buesa)
11. Re: staining of negative controls with IHC (AGrobe2555 <@t> aol.com)
12. RE: staining of negative controls with IHC (Tarango, Mark)
13. automated paraffin embedding systems (Lesley Bechtold)
14. Re: automated paraffin embedding systems (Rene J Buesa)
15. RE: automated paraffin embedding systems (Mike Pence)
16. RE: automated paraffin embedding systems (Lesley Bechtold)
17. Antibody cocktails (Christine I. Braaten)
18. RE: staining of negative controls with IHC (FU,DONGTAO)
----------------------------------------------------------------------
Message: 1
Date: Mon, 12 Nov 2007 10:14:18 -0800 (PST)
From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
Subject: [Histonet] Getting a Tissue Processor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <750683.40287.qm <@t> web62408.mail.re1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
My lab is FINALLY getting a Shandon Excelsior Tissue Processor. I was wondering if anyone had any good protocols for the machine that would be great for mouse tissue...mainly the GI trac and liver and kidneys.
Thanks for all of your help!
Jen
__________________________________________________
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------------------------------
Message: 2
Date: Mon, 12 Nov 2007 13:54:54 -0600
From: "Vancleave, Vince" <vvancleave <@t> hendrickhealth.org>
Subject: [Histonet] non pathologist grossing vs processing continued
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<740F77F6594C20458BDC2A4A500D8D41501642 <@t> EMAIL.hhs.hendrickhealth.org>
Content-Type: text/plain; charset="iso-8859-1"
From: Vancleave, Vince
Sent: Wed 11/7/2007 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Webster, John A. M.D.
Subject: Still not sure about this low complexity or not
"The thing you have to remember, Vince, is that CLIA and CAP are two
different, completely separate entities. CLIA is government controlled
and CAP is private. Whereas CAP recently decided to separate grossing
into two different complexities, CLIA has not. Bottom line...if you are
CLIA licensed you MUST follow the CLIA standard. If not, then you can do
whatever you like.
Charles Embrey Jr., PA(ASCP)"
Charles makes a great point but:
I'm just not sure I'm sold on this yet, b/c also in the CLIA regs it states in sec. 493.559 about exemption from CLIA, via using a private accreditation agency (which is what CAP is), and states clearly that this agency can only be deemed acceptable if their requirements for the laboratory are equivalent or more stringent and the laboratory would meet CLIA requirements if the laboratory had not been granted deemed status.
Ya'll are right, this is a mess!
So, wouldn't this essentially mean that according to CLIA, since CAP is an acceptable private agency, they are giving the ok for CAP's decision on this? Wouldn't CAP be unallowed to do what they do if it was less stringent than CLIA? I also find nothing in the entire CLIA 88' that indicates that this would not be ok. In fact, CLIA has a criteria for categorization of complexity: http://www.fda.gov/cdrh/clia/categorization.html . And in their database of tests that they have said has to absolutely be a certain way, I find no reference to tissue description, gross examination, tissue examination, or processing.
Have I stumped anyone yet, or is there some more things I am missing? I really appreciate all ya'lls willingness to re-visit this issue again. I guess I am a stuborn monkey!
Thanks,
Vince Van Cleave, BS, PA(ASCP), HTL, HT
Laboratory Manager and Pathologist Assistant
(325)670-6528
Clinical Pathology Associates
Abilene, TX
PRIVILEGED AND CONFIDENTIAL NOTICE:
The information contained in this e-mail may be confidential and/or privileged. This e-mail is intended to be reviewed initially by only the individual named above. If the reader of this e-mail is not the intended recipient or a representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail, or the information contained herein, is prohibited. If you have received this e-mail in error, notify the above sender, send the message back and then delete it. Thank you for your assistance.
Hendrick Health System 1900 Pine St. Abilene, Texas 79601 - 325.670.2000 79601 - 325.670.2000
------------------------------
Message: 3
Date: Mon, 12 Nov 2007 14:28:36 -0600
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] non pathologist grossing vs processing
continued
To: "Vancleave, Vince" <vvancleave <@t> hendrickhealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<44780C571F28624DBB446DE55C4D733A1FE56A <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
Hi Vince, Sorry to take so long in getting back to you but things here
have been very busy. By CLIA's guidelines it sounds as though CAP has
removed themselves as an "acceptable" private accreditation when they
loosened the restrictions on grossing. CLIA's database may not list
grossing but CLIA did publish a guideline to clarify their position:
This is the CLIA '88 guideline for high complexity testing personnel-
CLIA '88 493.1489
"On or before April 24 1995 (I) be a high school graduate or equivalent;
and (b) have documentation of training appropriate for the test
performed before analyzing patient specimens"...After that date it
requires an associate degree in a biological or chemical science or
medical laboratory technology -or- qualify as a medical technologist
with a bachelor's degree from an accredited institution -or- earned a
bachelor's degree in a chemical, physical, biologic or clinical
laboratory science.
A few years ago CLIA put out this interpretive guideline, (printed
below), to clear up the question of grossing. As you see, it references
493.1498 (the above "high complexity guideline"). They basically sum up
that if you examine/describe the tissue for record noting color weight
or measurement then it is grossing and falls under 493.1498. Tissue
processing as CAP calls it involves noting size and other
characteristics and qualifies as grossing under CLIA.
The new federal register CLIA interpretive guidelines appendix C subpart
M effective April 24, 2003 states in section 493.1461(e) "In the case if
gross examinations, the technical supervisor may delegate to individuals
qualified under 493.1498 the responsibility for the physical
examination/description, including color, weight, measurement, and other
characteristics of the tissue; or other mechanical procedures for which
a specific written protocol has been developed."
I hope this helps. I don't know which side will budge first but I have
heard whisperings of CLIA doing something in the near future but what
ever it is it will probably only muddy the water further. All I can say
is that CLIA is what happens when a government bureaucracy gets involved
with healthcare.
Charles Embrey PA(ASCP)
Histology Manager
Carle Clinic
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Vancleave, Vince
Sent: Monday, November 12, 2007 1:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] non pathologist grossing vs processing continued
From: Vancleave, Vince
Sent: Wed 11/7/2007 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Webster, John A. M.D.
Subject: Still not sure about this low complexity or not
"The thing you have to remember, Vince, is that CLIA and CAP are two
different, completely separate entities. CLIA is government controlled
and CAP is private. Whereas CAP recently decided to separate grossing
into two different complexities, CLIA has not. Bottom line...if you are
CLIA licensed you MUST follow the CLIA standard. If not, then you can do
whatever you like.
Charles Embrey Jr., PA(ASCP)"
Charles makes a great point but:
I'm just not sure I'm sold on this yet, b/c also in the CLIA regs it
states in sec. 493.559 about exemption from CLIA, via using a private
accreditation agency (which is what CAP is), and states clearly that
this agency can only be deemed acceptable if their requirements for the
laboratory are equivalent or more stringent and the laboratory would
meet CLIA requirements if the laboratory had not been granted deemed
status.
Ya'll are right, this is a mess!
So, wouldn't this essentially mean that according to CLIA, since CAP is
an acceptable private agency, they are giving the ok for CAP's decision
on this? Wouldn't CAP be unallowed to do what they do if it was less
stringent than CLIA? I also find nothing in the entire CLIA 88' that
indicates that this would not be ok. In fact, CLIA has a criteria for
categorization of complexity:
http://www.fda.gov/cdrh/clia/categorization.html . And in their
database of tests that they have said has to absolutely be a certain
way, I find no reference to tissue description, gross examination,
tissue examination, or processing.
Have I stumped anyone yet, or is there some more things I am missing? I
really appreciate all ya'lls willingness to re-visit this issue again.
I guess I am a stuborn monkey!
Thanks,
Vince Van Cleave, BS, PA(ASCP), HTL, HT
Laboratory Manager and Pathologist Assistant
(325)670-6528
Clinical Pathology Associates
Abilene, TX
PRIVILEGED AND CONFIDENTIAL NOTICE:
The information contained in this e-mail may be confidential and/or
privileged. This e-mail is intended to be reviewed initially by only the
individual named above. If the reader of this e-mail is not the intended
recipient or a representative of the intended recipient, you are hereby
notified that any review, dissemination or copying of this e-mail, or
the information contained herein, is prohibited. If you have received
this e-mail in error, notify the above sender, send the message back and
then delete it. Thank you for your assistance.
Hendrick Health System 1900 Pine St. Abilene, Texas 79601 -
325.670.2000 79601 - 325.670.2000
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 12 Nov 2007 16:52:19 -0600
From: SHargrove <@t> urhcs.org
Subject: [Histonet] Susie Hargrove is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFB02FBAC8.AEE972E9-ON86257391.007DA3B0-86257391.007DA3B0 <@t> urhcs.org>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 11/12/2007 and will not return until
11/19/2007.
I will respond to your message when I return.
------------------------------
Message: 5
Date: Tue, 13 Nov 2007 10:27:51 +1100
From: "Michelle McDonald" <MichelM9 <@t> chw.edu.au>
Subject: RE: [Histonet] deplasticizing PMMA sections for staining
To: "Monfils, Paul" <PMonfils <@t> Lifespan.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <47BD6E7614A693499453835D47E36F7003882B4F <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="us-ascii"
We actually use Acetone to deplasticise MMA sections. Only takes 2
changes for 10 mins each.
Michelle
Dr Michelle McDonald B.MedSci, PhD
Research Officer
Orthopaedic Research Dept.
The Children's Hospital Westmead.
Westmead NSW 2145
Australia
Ph. +612 9845 1451
Fax. +612 9845 3078
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Tuesday, 6 November 2007 6:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] deplasticizing PMMA sections for staining
In my experience, xylene is a rather poor solvent for PMMA. It works
much faster at 60 degrees, but don't try heating flammable solvents in
your oven unless you are certain it is certified explosion-proof (most
paraffin ovens are not). Even then, unless you have the oven in or at
least next to a fume hood, you'll get a lot of xylene vapors in the air
as a result of heating it. I deplasticise PMMA with 2-methoxyethyl
acetate, which only takes a couple of hours at room temperature. This
has a rather strong though not unpleasant odor, and should be used in
the hood.
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 6
Date: Tue, 13 Nov 2007 09:34:47 +0800
From: "San Tin" <san_tin <@t> biolab.com.sg>
Subject: [Histonet] MyoD1
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1194976800.24285.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Dear All,
Can anyone give me the protocol of MyoD1 clone 5.8A on Ventana IHC
autostainer? I have problem with staining.
Thanks
San
------------------------------
Message: 7
Date: Tue, 13 Nov 2007 14:03:54 +1100
From: "Farish, Craig" <cfarish <@t> csu.edu.au>
Subject: [Histonet] RE: TGIF
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<79A000B60AFE8045BA2581119DFEC44402A251D4 <@t> ESWW01.CSUMain.csu.edu.au>
Content-Type: text/plain; charset="us-ascii"
And in return:
A surgeon, pathologist, physician, psychiatrist and radiologist go duck
hunting out on a pond.
As the first duck flies over head, the psychiatrist takes aim, ....but
doesn't fire.
"What the hell happened?" demands the surgeon (they're known for their
retiring manner)
"Well, says the psychiatrist, I know its a duck, and YOU know its a duck
- but does the duck know its a duck?"
The second bird flies over head, and the physician takes aim.....but
doesn't fire.
"What now???" roars the surgeon.
Well, says the physician, in a recent article I read in the New England
Journal of Medicine, 12% of ducks are actually
Terns, and they're a protected species....
The third duck flies over, and the radiologist takes aim......then
paddles the boat further into the pond, then takes aim, then paddles
back a bit, then takes a further aim....and by this time the duck has
gone.
"Jesus wept!" explodes the surgeon, what the f*** were you doing?
Well, says the radiologist - every time I took aim, I realised that I
needed another view.
After lunch, they re-group for more hunting.
This time the surgeon gets out his double barrelled, gold plated
bazooka, blows his duck whistle - and as a flock of birds swarm over
head, he fires randomly and enthusiastically into the air. Objects rain
out of the sky. When he had finally finished blasting away to the
heavens - he turned to the pathologist and said: "row over to all those
bodies, and tell me if any of them was a duck"
Craig (Joe) Farish
Technical Officer
School of Agricultural and Veterinary Sciences
Charles Sturt University
Wagga Wagga NSW
Australia
<mailto:cfarish <@t> csu.edu.au> ' I don't want to achieve immortality
through my work, I want to achieve immortality through not dying' -
Woody Allen
------------------------------
Message: 8
Date: Tue, 13 Nov 2007 12:54:35 +0200
From: "K M" <ombadda3 <@t> gmail.com>
Subject: [Histonet] Claudin-1 as a prognostic factor in colorectal
cancer
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<ac7603c00711130254w17d15d33p4b71d20185895ffd <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi all
I am here again and I start do my Msc and the topic I have chosen is:
Claudin-1 as a prognostic factor in colorectal cancer.
I will tell you each step I will do so I can benefit from your
valuable experiences and knowledge.
Thanks again to all members of this valuable forum
--
Khalaf
Mobile Phone: 0020127920404
Egypt
------------------------------
Message: 9
Date: Tue, 13 Nov 2007 08:18:06 -0500
From: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>
Subject: [Histonet] staining of negative controls with IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <47395DBE.6EA2.00FD.0 <@t> vetmed.ufl.edu>
Content-Type: text/plain; charset=US-ASCII
We are currently retrieving animal tissue in a pressure cooker with a program of 125 degrees for 30 secs. This protocol was taken from biocare but I'm sure this is for human tissue. Most of the tissue is retrieved with Biocare's Reveal (pH 6.0). We are receiving some background staining of our negative controls whereas if we did not retrieve them, there is none. We were staining for KI-67 which is a nuclear stain. I am using a tonsil for the control. The negative control showed some sporatic cytoplasmic staining on the Universal negative control from biocare as well as deionized water. Since then we have taken the temperature down to 115 degrees and increased the time to 25 minutes. It seems to have decreased the cytoplasmic staining but there is still some lingering. Anyone out there have any ideas??? I'm sure there is a better protocol for retrieving animal tissues. It has to be a time and temperature thing!
MaryAnn Dixon
Biological Scientist
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4517
------------------------------
Message: 10
Date: Tue, 13 Nov 2007 05:32:35 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] staining of negative controls with IHC
To: MaryAnn Dixon <DixonM <@t> vetmed.ufl.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <428387.48113.qm <@t> web61222.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
There are many who prefer the pressure cooker because of the low incubation period but I personally did not like it because of background issues as you describe and because some tissues peel-off the slides (too much heat).
Because of that I ended using a HIER step in a buffer (citrate or EDTA depending on the pH) initially heated to boliling temperature in a microwave oven and the actual HIER to take place in a steamer during 20 minutes + 20 minutes out of the steamer. All problems were resolved this way. Give it a try.
René J.
MaryAnn Dixon <DixonM <@t> vetmed.ufl.edu> wrote:
We are currently retrieving animal tissue in a pressure cooker with a program of 125 degrees for 30 secs. This protocol was taken from biocare but I'm sure this is for human tissue. Most of the tissue is retrieved with Biocare's Reveal (pH 6.0). We are receiving some background staining of our negative controls whereas if we did not retrieve them, there is none. We were staining for KI-67 which is a nuclear stain. I am using a tonsil for the control. The negative control showed some sporatic cytoplasmic staining on the Universal negative control from biocare as well as deionized water. Since then we have taken the temperature down to 115 degrees and increased the time to 25 minutes. It seems to have decreased the cytoplasmic staining but there is still some lingering. Anyone out there have any ideas??? I'm sure there is a better protocol for retrieving animal tissues. It has to be a time and temperature thing!
MaryAnn Dixon
Biological Scientist
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4517
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Never miss a thing. Make Yahoo your homepage.
------------------------------
Message: 11
Date: Tue, 13 Nov 2007 10:39:46 EST
From: AGrobe2555 <@t> aol.com
Subject: Re: [Histonet] staining of negative controls with IHC
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <c44.23371cd1.346b1f42 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
You don't say what detection system you are using....However, if you are
using a Biotin-avidin/streptavidin detection, the background may be due to the
exposure of endogenous biotin by HIER. You may need to incorporate an
appropriate blocking agent/system
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
************************************** See what's new at http://www.aol.com
------------------------------
Message: 12
Date: Tue, 13 Nov 2007 08:24:00 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] staining of negative controls with IHC
To: "MaryAnn Dixon" <DixonM <@t> vetmed.ufl.edu>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<5AEC610C1CE02945BD63A395BA763EDE011B6F9A <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii
You probably don't even need to get up to boiling for ki-67. Try 30
minutes at 95 C in your pressure cooker. Even on a "negative" control
you're bound to see something stain positive for ki-67, since it's a
proliferation marker... although it shouldn't be cytoplasmic. I'm just
guessing there's got to be a cell or two in the tissue that's dividing.
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of MaryAnn
Dixon
Sent: Tuesday, November 13, 2007 5:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staining of negative controls with IHC
We are currently retrieving animal tissue in a pressure cooker with a
program of 125 degrees for 30 secs. This protocol was taken from
biocare but I'm sure this is for human tissue. Most of the tissue is
retrieved with Biocare's Reveal (pH 6.0). We are receiving some
background staining of our negative controls whereas if we did not
retrieve them, there is none. We were staining for KI-67 which is a
nuclear stain. I am using a tonsil for the control. The negative control
showed some sporatic cytoplasmic staining on the Universal negative
control from biocare as well as deionized water. Since then we have
taken the temperature down to 115 degrees and increased the time to 25
minutes. It seems to have decreased the cytoplasmic staining but there
is still some lingering. Anyone out there have any ideas??? I'm sure
there is a better protocol for retrieving animal tissues. It has to be a
time and temperature thing!
MaryAnn Dixon
Biological Scientist
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4517
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
"EMF <nvcancer.org>" made the following annotations.
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------------------------------
Message: 13
Date: Tue, 13 Nov 2007 11:01:02 -0500
From: "Lesley Bechtold" <lesley.bechtold <@t> jax.org>
Subject: [Histonet] automated paraffin embedding systems
To: "Histology Network" <histonet <@t> Pathology.swmed.edu>
Message-ID: <20071113110102345.00000002152 <@t> spikey>
Content-Type: text/plain; charset=us-ascii
Hi,
I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
------------------------------
Message: 14
Date: Tue, 13 Nov 2007 09:03:48 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] automated paraffin embedding systems
To: Lesley Bechtold <lesley.bechtold <@t> jax.org>, Histology Network
<histonet <@t> Pathology.swmed.edu>
Message-ID: <284972.24114.qm <@t> web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
This embedding instrument is manufactured by Sakura. It is not that the instrument orients the tissue, that is done when the tissues are placed in the cassette and held in place by a plastic frame that will be sectioned along with the tissue when the block is prepared. To function ideally it requires a special type of cassette and cassette holder (that is used alsao by the Sakura Xpress microwave tissue processor).
René J.
Lesley Bechtold <lesley.bechtold <@t> jax.org> wrote:
Hi,
I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
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Message: 15
Date: Tue, 13 Nov 2007 11:09:57 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] automated paraffin embedding systems
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "Lesley Bechtold"
<lesley.bechtold <@t> jax.org>, "Histology Network"
<histonet <@t> Pathology.swmed.edu>
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C7A8 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="iso-8859-1"
Is anyone out there using this function of the Sakura Xpress?
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 13, 2007 11:04 AM
To: Lesley Bechtold; Histology Network
Subject: Re: [Histonet] automated paraffin embedding systems
This embedding instrument is manufactured by Sakura. It is not that the instrument orients the tissue, that is done when the tissues are placed in the cassette and held in place by a plastic frame that will be sectioned along with the tissue when the block is prepared. To function ideally it requires a special type of cassette and cassette holder (that is used alsao by the Sakura Xpress microwave tissue processor).
René J.
Lesley Bechtold <lesley.bechtold <@t> jax.org> wrote:
Hi,
I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. _______________________________________________
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------------------------------
Message: 16
Date: Tue, 13 Nov 2007 12:26:32 -0500
From: "Lesley Bechtold" <lesley.bechtold <@t> jax.org>
Subject: RE: [Histonet] automated paraffin embedding systems
To: "Mike Pence" <mpence <@t> grhs.net>, "Rene J Buesa"
<rjbuesa <@t> yahoo.com>, "Histology Network"
<histonet <@t> Pathology.swmed.edu>
Message-ID: <20071113122632276.00000002152 <@t> spikey>
Content-Type: text/plain; charset=iso-8859-1
Thank you to everyone for so quickly responding!!! I'm calling my Sakura rep for more info.
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
-----Original Message-----
From: Mike Pence [mailto:mpence <@t> grhs.net]
Sent: Tuesday, November 13, 2007 12:10 PM
To: Rene J Buesa; Lesley Bechtold; Histology Network
Subject: RE: [Histonet] automated paraffin embedding systems
Is anyone out there using this function of the Sakura Xpress?
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 13, 2007 11:04 AM
To: Lesley Bechtold; Histology Network
Subject: Re: [Histonet] automated paraffin embedding systems
This embedding instrument is manufactured by Sakura. It is not that the instrument orients the tissue, that is done when the tissues are placed in the cassette and held in place by a plastic frame that will be sectioned along with the tissue when the block is prepared. To function ideally it requires a special type of cassette and cassette holder (that is used alsao by the Sakura Xpress microwave tissue processor).
René J.
Lesley Bechtold <lesley.bechtold <@t> jax.org> wrote:
Hi,
I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?
Thank you!
Lesley
Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences
The Jackson Laboratory
600 Main St.
Bar Harbor, ME 04609
207-288-6322
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Be a better pen pal. Text or chat with friends inside Yahoo! Mail. See how. _______________________________________________
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------------------------------
Message: 17
Date: Tue, 13 Nov 2007 12:32:49 -0500
From: "Christine I. Braaten" <CBraaten <@t> Cheshire-Med.COM>
Subject: [Histonet] Antibody cocktails
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F644CD64B2313F43BB3D2496B454CDACAC1E01 <@t> CMC-EX01.cheshire-med.com>
Content-Type: text/plain; charset="us-ascii"
Can anybody tell me if there is another company besides Biocare that
offers an antibody cocktail with P504S, HMW CK, and p63? Does anybody
use the Biocare antibody detection system? I'd appreciate some feedback.
Thanks.
CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message.
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Message: 18
Date: Tue, 13 Nov 2007 12:40:56 -0500 (EST)
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
Subject: RE: [Histonet] staining of negative controls with IHC
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1798590190.34131194975656578.JavaMail.osg <@t> osgjas03.cns.ufl.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Hi,
Ki67 staining in both human and mouse tissue in our lab works
very well. We use steamer method 30min in Citra buffer.
Ann Dongtao Fu MD, Ph.D
Lab Manager
Molecular Pathology Core
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610
On Tue Nov 13 11:24:00 EST 2007, "Tarango, Mark"
<mtarango <@t> nvcancer.org> wrote:
> You probably don't even need to get up to boiling for ki-67. Try
> 30
> minutes at 95 C in your pressure cooker. Even on a "negative"
> control
> you're bound to see something stain positive for ki-67, since
> it's a
> proliferation marker... although it shouldn't be cytoplasmic.
> I'm just
> guessing there's got to be a cell or two in the tissue that's
> dividing.
>
>
> Mark Adam Tarango HT(ASCP)
> Histology & IHC Supervisor
> Nevada Cancer Institute
> One Breakthrough Way
> Las Vegas, NV 89135
> Direct Line (702) 822-5112
> Treo (702) 759-9229
> Fax (702) 939-7663
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> MaryAnn
> Dixon
> Sent: Tuesday, November 13, 2007 5:18 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] staining of negative controls with IHC
>
> We are currently retrieving animal tissue in a pressure cooker
> with a
> program of 125 degrees for 30 secs. This protocol was taken from
> biocare but I'm sure this is for human tissue. Most of the tissue
> is
> retrieved with Biocare's Reveal (pH 6.0). We are receiving some
> background staining of our negative controls whereas if we did
> not
> retrieve them, there is none. We were staining for KI-67 which
> is a
> nuclear stain. I am using a tonsil for the control. The negative
> control
> showed some sporatic cytoplasmic staining on the Universal
> negative
> control from biocare as well as deionized water. Since then we
> have
> taken the temperature down to 115 degrees and increased the time
> to 25
> minutes. It seems to have decreased the cytoplasmic staining but
> there
> is still some lingering. Anyone out there have any ideas??? I'm
> sure
> there is a better protocol for retrieving animal tissues. It has
> to be a
> time and temperature thing!
> MaryAnn Dixon
> Biological Scientist
> Anatomic Pathology
> University of Florida
> School of Veterinary Medicine
> 352-392-2235 ext 4517
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> "EMF <nvcancer.org>" made the following annotations.
> ------------------------------------------------------------------------------
> CONFIDENTIALITY NOTICE: This e-mail message, including any
> attachments, is for the sole use of the intended recipient(s) and
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> message or its attachments. If you believe you have received this
> e-mail message in error, please contact the sender by reply
> e-mail and destroy all copies of the original message
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>
>
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>
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