[Histonet] Special Stainers Becky Johnson

[Joyce Cline]

Becky,

Our first stainer we had for 10 years, we received a new one this year.
Ventana told us the pH should be 5.5 to 6.0 (-or+ .1). We check the pH
of our special stain wash when we mix it, not at each run.

-----Original Message-----
From: Rebecca Johnson [mailto:raj <@t> bluemarble.net] 
Sent: Thursday, November 15, 2007 9:05 PM
To: Joyce Cline
Subject: Re: [Histonet] Special Stainers Becky Johnson

Joyce,
We have always done everything Ventana told us to do from the very 
beginning. We use the plus green slides that Ventana advised. Daily
before 
any run is started the Ph is taken on the special stain wash., the level
is 
checked and the liquid coverslip, special stain wash filled then the
machine 
is primed to clear all lines from any air or bubbles. The clean cycle is

done when special stains are finished for the day.  What has Ventana
told 
you the Ph should be on the special stain wash?  We only have one Nexus 
stainer and it is several years old, but the maintance has always been
done 
regular.  We have had the Benchmark XT for about a year.  So far no
problems 
with it.  Thanks for all your advice.
Becky
----- Original Message ----- 
From: "Joyce Cline" <jcline <@t> wchsys.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, November 15, 2007 1:43 PM
Subject: RE: [Histonet] Special Stainers Becky Johnson


> Hi Becky,
> I use a Ventana stainer and I'll pass along a few hints that we have
> learned. For silver stains we use only Superfrost plus slides. I have
> tried some of the others but the staining quality was not adequate. We
> also leave our slides in the oven (72C) for 1/2 hour. We use the
> cleaning kit after every run.
> I don't know if you have tried any of these hints but if not, I hope
> they help.
> -----Original Message-----
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Rebecca
> Johnson
> Sent: Wednesday, November 14, 2007 6:23 PM
> To: histonet
> Subject: [Histonet] Special Stainers
>
> I need to know what everyone is using for Special Stainers?  I now
have
> Ventana special stainer. Have had the same machine for several years
> with maintance(cleaning)  done on it daily, monthly and PM.  Just not
> real happy with the service and inconsistent staining with the silver
> stains.  Thanks for all your info.
> Becky
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