[Histonet] Matrigel as culture medium

Johnson, Teri TJJ <@t> Stowers-Institute.org
Fri Nov 16 09:57:16 CST 2007

One of our researchers is using matrigel as a culture medium on
chambered slides. We tried paraffin embedding them, and found the
Matrigel wasn't really solid enough to hold the cellular structure in
place. We found it particularly difficult to keep some sort of
consistency scraping it off (it had the consistency of warm jelly).

I'd love to hear some ideas on how we can accomplish sample processing
without centrifugation. Ideally it would be good to encase the cells in
a matrix that would solidfy enough to embed as a cell block, keeping the
cellular structure intact. 

Thanks for your help!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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