[Histonet] Tape coverslipper
Rene J Buesa
rjbuesa <@t> yahoo.com
Thu Nov 15 13:21:09 CST 2007
Usually bubbles trapped underneath the tape are due to less than adequate amount of xylene. You have to regulate the size of the xylene drop to the amount needed to cover without bubbles.
Don't overdue it because you may end with toomuch xylene and very "wet" slides.
Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com> wrote:
I love our automatic tape coverslipper. I'm a new user of an old machine,
and I've noticed a problem. Perhaps someone can offer some insight.
Occasionally, an artefact appears that resembles trapped air, microscopic
bubbles, if you will, that appear gray-black on a higher plane than the
tissue, so I know it's a superficial artefact. I just don't know what is
causing it - but I'm sure it has something to do with the tape
coverslipper - but what? I don't know enough about the machine to
troubleshoot it - it seems a relatively simple instrument. Tape, a wheel
and xylene. Where are the bubbles coming from, and how do I get rid of
them? The pathologist thought it was some kind of dirt or dust debris
accumulation - but we've decided it's air. Help.
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