[Histonet] RE: Histonet Digest, Vol 48, Issue 21

[Joseph Nerk]

Does anyone in Histoland provide any information on the Cryotome E or SME manufactured by Shandon. I am in the process of purchasing a new Cryostat. so need some feedback.



> From: histonet-request <@t> lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 48, Issue 21> To: histonet <@t> lists.utsouthwestern.edu> Date: Thu, 15 Nov 2007 10:00:16 -0800> > Send Histonet mailing list submissions to> histonet <@t> lists.utsouthwestern.edu> > To subscribe or unsubscribe via the World Wide Web, visit> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to> histonet-request <@t> lists.utsouthwestern.edu> > You can reach the person managing the list at> histonet-owner <@t> lists.utsouthwestern.edu> > When replying, please edit your Subject line so it is more specific> than "Re: Contents of Histonet digest..."> > > Today's Topics:> > 1. WG: [Histonet] MyoD1 (Gudrun Lang)> 2. tissue processors (Jennifer Sipes)> 3. Hi all (Kimberly Secrest)> 4. RE: microtomy technical question (Monson, Frederick )> 5. Re: Giemsa Stain (Robert Richmond)> 6. pepsin (MKing)> 7. Re: pepsin (Joanne Mauger)> 8. RE: Colored OCT (Norton, Sally)> 9. Colored OCT (Webb, Dorothy L)> 10. Re: Giemsa Stain (Bill)> 11. Gill's III stainer question (karenadams <@t> comcast.net)> > > ----------------------------------------------------------------------> > Message: 1> Date: Thu, 15 Nov 2007 16:12:16 +0100> From: "Gudrun Lang" <gu.lang <@t> gmx.at>> Subject: WG: [Histonet] MyoD1> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID: <001601c82799$e94a2850$6412a8c0 <@t> dielangs.at>> Content-Type: text/plain; charset="iso-8859-1"> > > 1:30, cc1 32 min, 32 min ink., 37°C > > Gudrun Lang> > Biomed. Analytikerin> Histolabor> Akh Linz> Krankenhausstr. 9> 4020 Linz> +43(0)732/7806-6754> -----Ursprüngliche Nachricht-----> Von: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von San Tin> Gesendet: Dienstag, 13. November 2007 02:35> An: histonet <@t> lists.utsouthwestern.edu> Betreff: [Histonet] MyoD1> > Dear All,> > Can anyone give me the protocol of MyoD1 clone 5.8A on Ventana IHC> autostainer? I have problem with staining.> > > > Thanks> > San> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > ------------------------------> > Message: 2> Date: Thu, 15 Nov 2007 07:19:13 -0800 (PST)> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>> Subject: [Histonet] tissue processors> To: histonet <@t> lists.utsouthwestern.edu> Message-ID: <98496.35382.qm <@t> web62411.mail.re1.yahoo.com>> Content-Type: text/plain; charset=us-ascii> > I was wondering if anyone had a good protocol for mouse tissue on a Shandon Excelsior Tissue Processor.> > Thanks> > Jennifer K. Sipes, ALAT > Sr. Laboratory Technician > Johns Hopkins University > Ross 929 > 720 Rutland Avenue > Baltimore, MD 21205 > phone: 410-614-0131 > 410-955-9688 > fax: 410-955-9677 > cell: 443-631-6361 > e-mail: jsipes1 <@t> jhmi.edu> > > ____________________________________________________________________________________> Be a better pen pal. > Text or chat with friends inside Yahoo! Mail. See how. http://overview.mail.yahoo.com/> > ------------------------------> > Message: 3> Date: Thu, 15 Nov 2007 10:37:02 -0500> From: "Kimberly Secrest" <ksecrest <@t> hsc.wvu.edu>> Subject: [Histonet] Hi all> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID: <473C2154.C068.0078.0 <@t> hsc.wvu.edu>> Content-Type: text/plain; charset=US-ASCII> > Does anybody have or know of anyone willing to donate histology equipment (i.e. microtomes, tissue processor, embedding center, routine stainer, etc) or supplies (i.e. slides, cassettes, reagents) for the start up of a histotechnology program?> > > Kimberly Secrest, HTL, QIHC> Instructor> Department of Pathology> School of Medicine> West Virginia University> > > > > > ------------------------------> > Message: 4> Date: Thu, 15 Nov 2007 10:57:31 -0500> From: "Monson, Frederick " <FMonson <@t> wcupa.edu>> Subject: RE: [Histonet] microtomy technical question> To: <histonet <@t> lists.utsouthwestern.edu>> Message-ID:> <641CEFFC7E5B6C42AB59539653FD082304A90A58 <@t> wcu-ex-emp2.PASSHE.LCL>> Content-Type: text/plain; charset="us-ascii"> > I never respond without an answer from outside the bag, so to speak.> > 1. I once was quite ill (at the age of 40) and received the news that> my CBC required a bone marrow follow up. I asked if the differential> was at fault and heard a 'Yes'. I asked if the differential was> performed manually. The answer was 'No'. I demanded a manual> differential. The bone marrow was cancelled. Don't trust machines too> far is one of my life lessons.> > 2. I never had to use watered or iced paraffin blocks BEFORE I was> required by volume to process tissues in an auto processor. Prior to> using auto, I had very early on learned the consequences of> over-dehydrating, over-clearing and especially overcooking my specimens> and avoided doing so. Now with so many 110V AC lines measuring 128V AC> or <110V AC on occasion (the latter does no harm to the tissue but> lengthen the times in each bath), why is there a question about problems> with the outcomes?> > 3. For my SEM, I have a $5,600 Uninterruptible Power Supply (UPS) that> regulates the AC to within the specs of my instrument so that I am not> at the mercy of PECO - our local supplier - when I run long-duration> automated analyses.> > Most institutions are treated by the power companies as industrial> customers. The consequence is that power fluctuations are much more> frequent. Industry compensates by investing in regulation wherever it> is required. Institutions, on the other hand, are often ignorant of how> much or little power fluctuations affect performance in various> organizational modules - like laboratories in which analyses are> performed. One will not see the difference between 128 and 108 in the> performance of the coffee maker. Overnight, no one is watching and> monitoring.> > Hope this helps,> > Fred Monson> > Frederick C. Monson, PhD> Technical Director> Microanalysis and Imaging Research and Training Center (MIRTC)> Large Scientific Instrument Core> Geology, West Chester University> S. Church St. and W. Rosedale Ave.> West Chester, PA, 19320> 610-738-0437> fmonson <@t> wcupa.edu> New Scheduler: http://lexspiac.wcupa.edu/cgi-bin/ureserve_gold.pl> Web Page: http://lexspiac.wcupa.edu> > > > > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva C> Andersson> Sent: Tuesday, November 13, 2007 4:32 PM> To: histonet <@t> lists.utsouthwestern.edu> Subject: [Histonet] microtomy technical question> > Hello everyone,> I have some questions regarding microtomy. > We place our blocks face down on ice. The problem is that some tissues> take a very long time before they are ready to be cut(some more than 6> hours). For some tissues like kidney samples (mouse tissue) we have been> using glycerol on the ice. This does seem to cut down on the time> needed. My question is which other tissues can I use this technique on?> Do you have any other suggestions for how to get the tissue hydrated for> cutting?> Thank you for your help,> Eva Permaul> Georgetown University> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ------------------------------> > Message: 5> Date: Thu, 15 Nov 2007 11:13:12 -0500> From: "Robert Richmond" <RSRICHMOND <@t> aol.com>> Subject: [Histonet] Re: Giemsa Stain> To: histonet <@t> lists.utsouthwestern.edu> Message-ID:> <abea52a60711150813y16b23b6bj862aa6e232a5434d <@t> mail.gmail.com>> Content-Type: text/plain; charset=ISO-8859-1> > Bill (good ol' Bill - where?) says of staining Helicobacter:> > >>I like Alcian yellow better, but we have trouble getting it. Anyone> know of a good, reliable source in the US? I think IHC is overkill and> too expensive.<<> > The original chemical synthesis of Alcian yellow is not> environmentally safe, though the dye may still be made in countries> that are not concerned about such matters. Dick Dapson at Anatech told> us some time ago that he had achieved an environmentally acceptable> synthesis of Alcian yellow, but that the product did not have a> satisfactory shelf life. Anatech offers a substitute for Alcian> yellow, which I have not seen.> > (I have no connection with Anatech.)> > Bob Richmond> Samurai Pathologist> Knoxville TN> > > > ------------------------------> > Message: 6> Date: Thu, 15 Nov 2007 11:29:36 -0500> From: MKing <making <@t> ufl.edu>> Subject: [Histonet] pepsin> To: histonet <@t> lists.utsouthwestern.edu> Message-ID: <473C73F0.3010607 <@t> ufl.edu>> Content-Type: text/plain; charset=ISO-8859-1; format=flowed> > An antibody vendor recommended pepsin pretreatment prior to primary > antibody incubation of our free-floating 40 um formaldehyde-fixed rat > brain frozen sections, but provided a protocol that only listed pepsin > by weight. Since enzyme activity ranges from 3 to 4500 units/mg in > commercial sources, does anyone have a rough idea what kind of activity > is sufficient without destroying the tissue (which is what happened with > the first test). Anybody find this approach worthwhile with similar tissue?> Thanks,> Mike King> UF Pharmacology & Therapeutics> > > > ------------------------------> > Message: 7> Date: Thu, 15 Nov 2007 11:44:01 -0500> From: "Joanne Mauger" <mauger <@t> email.chop.edu>> Subject: Re: [Histonet] pepsin> To: <histonet <@t> lists.utsouthwestern.edu>,<making <@t> ufl.edu>> Message-ID: <s73c310f.085 <@t> email.chop.edu>> Content-Type: text/plain; charset=US-ASCII> > Mike,> > We use pepsin at 2mgs/ml for digestion of formalin fixed, paraffin> embedded tissue. We use it at 37C for 10 to 30 minutes. We buy powdered> pepsin from Dako #S3002. > > Don't know what will happen to frozen tissue.> > Good luck,> > Jo Mauger> > > > ------------------------------> > Message: 8> Date: Thu, 15 Nov 2007 08:42:46 -0800> From: "Norton, Sally" <sally.norton <@t> seattlechildrens.org>> Subject: RE: [Histonet] Colored OCT> To: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>,> laurie.colbert <@t> huntingtonhospital.com,> histonet <@t> lists.utsouthwestern.edu, mickie25 <@t> netzero.net> Message-ID:> <D601281FC7E5BB47B5A89656DEFA0E73067849D6 <@t> s107.childrens.sea.kids>> Content-Type: text/plain; charset=us-ascii> > I have to ask the question. For what purpose do you use colored OCT?> We here, at Children's in Seattle, have never heard of it.> > Thank you.> Sally Norton, HT> Children's Hospital and Regional Medical Center> Seattle, WA> > > -----Original Message-----> From: histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robyn> Vazquez> Sent: Thursday, November 15, 2007 6:29 AM> To: laurie.colbert <@t> huntingtonhospital.com;> histonet <@t> lists.utsouthwestern.edu; mickie25 <@t> netzero.net> Subject: RE: [Histonet] Colored OCT> > Mickey,> We like lots of color so we use 4-6 drops, depending on the color we> want to achieve. BRIGHT COLORS!! And the variety of colors! It is> amazing the little things that make us happy!!!> > Robyn> OHSU> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.> > > > > > ------------------------------> > Message: 9> Date: Thu, 15 Nov 2007 11:06:11 -0600> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>> Subject: [Histonet] Colored OCT> To: histonet <@t> lists.utsouthwestern.edu> Message-ID:> <0E394B648E5284478A6CCB78E5AFDA270563528D <@t> hpes1.HealthPartners.int>> Content-Type: text/plain; charset="us-ascii"> > TBS carries the colored OCT. The order number is H-TFM and is> available in red or blue.> > Dorothy Webb, HT (ASCP)> Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962> Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care> ________________________________________> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.> > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.> > > ------------------------------> > Message: 10> Date: Thu, 15 Nov 2007 11:27:14 -0600> From: Bill <bill501 <@t> mindspring.com>> Subject: [Histonet] Re: Giemsa Stain> To: histonet <@t> lists.utsouthwestern.edu> Message-ID: <p06240800c3623177cd6d@[4.244.180.44]>> Content-Type: text/plain; charset="us-ascii"> > At 11:13 AM -0500 11/15/07, Robert Richmond wrote:> >Bill (good ol' Bill - where?) says of staining Helicobacter:> > I wondered why. Thanks Bob.> > > -- > _____________________________> Bill Blank> http://kernunnos.com (Celtic studies and numismatics)> OBOD's Message board: http://www.druidry.org/board/dhp/> > > > > > > ------------------------------> > Message: 11> Date: Thu, 15 Nov 2007 17:44:56 +0000> From: karenadams <@t> comcast.net> Subject: [Histonet] Gill's III stainer question> To: histonet <@t> lists.utsouthwestern.edu> Message-ID:> <111520071744.2924.473C859800022C3F00000B6C22070206539C030E0B0E020A9D0E05 <@t> comcast.net>> > Content-Type: text/plain> > > We are presently using Gill's III manually for H & E and on the automated stainer.....I am seeing mucin lightly staining on the automated stainer, but not the manual.....any thoughts??> --> Karen Adams > Supervisor> Pathology Laboratories West > 9303 Park West Blvd > Knoxville, TN 37923 > (865) 690-2111 FAX (865) 691-1623> > ------------------------------> > _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > End of Histonet Digest, Vol 48, Issue 21> ****************************************
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