[Histonet] Pin4

[Webb, Dorothy L]

Cell marque also carries the cocktail!!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, November 14, 2007 7:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 48, Issue 18


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Today's Topics:

   1. Re: Antibody cocktails (godsgalnow <@t> aol.com)
   2. Histology equipments (Ms. K?tia Cristina Catunda)
   3. Re: staining of negative controls with IHC (Johnson, Teri)
   4. processing (Webb, Dorothy L)
   5. RE: processing (Tarango, Mark)
   6. RE: processing (Tom McNemar)
   7. Histonet down? (Thomas Jasper)
   8. Re: Histology equipments (Rene J Buesa)
   9. RE: Antibody cocktails (Barry Madigan)
  10. microtomy technical question (Eva C Andersson)
  11. Re: microtomy technical question (Rene J Buesa)
  12. It may be more than just a microtomy technical question
      (Gayle Callis)
  13. Giemsa Stain (Schaundra Walton)
  14. RE: automated paraffin embedding systems
      (Bartlett, Jeanine (CDC/CCID/NCZVED))
  15. RE: automated paraffin embedding systems
      (Bartlett, Jeanine (CDC/CCID/NCZVED))
  16. Re: Giemsa Stain (Rene J Buesa)
  17. RE: Giemsa Stain (Marshall Terry Dr,	Consultant Histopathologist)
  18. RE: Giemsa Stain (Rene J Buesa)
  19. RE: Giemsa Stain (Kemlo Rogerson)
  20. Re: Giemsa Stain (koellingr <@t> comcast.net)
  21. RE: Giemsa Stain (Marshall Terry Dr,	Consultant Histopathologist)
  22. RE: Giemsa Stain (Marshall Terry Dr,	Consultant Histopathologist)
  23. RE: Giemsa Stain (Kemlo Rogerson)
  24. RE: Giemsa Stain (Rene J Buesa)


----------------------------------------------------------------------

Message: 1
Date: Tue, 13 Nov 2007 13:06:20 -0500
From: godsgalnow <@t> aol.com
Subject: Re: [Histonet] Antibody cocktails
To: CBraaten <@t> Cheshire-Med.COM, histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C9F437075F6496-EC4-5B3F <@t> WEBMAIL-MB20.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


Christine,



My understanding is that other vendors do have something similar to the cocktail, but they only contain 2 of the antibodies and the third has to be added at another step, not completely sure on that.?



But I do use the BioCare PIN-4 cocktail and have been doing so for 3 years now with no complaints; I?have done?a few thousands of these so far.? I use their Mach 2 Double Stain detection system with the cocktail and it works like a beaut.

Roxanne Soto HT(ASCP)QIHC


-----Original Message-----
From: Christine I. Braaten <CBraaten <@t> Cheshire-Med.COM>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, 13 Nov 2007 12:32 pm
Subject: [Histonet] Antibody cocktails




Can anybody tell me if there is another company besides Biocare that offers an antibody cocktail with P504S, HMW CK, and p63? Does anybody use the Biocare antibody detection system? I'd appreciate some feedback. Thanks.


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Message: 2
Date: Tue, 13 Nov 2007 16:20:18 -0300
From: Ms. K?tia Cristina Catunda 	<katia.catunda <@t> cipax.com.br>
Subject: [Histonet] Histology equipments
To: "'Histology Network'" <histonet <@t> Pathology.swmed.edu>
Message-ID:
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Hi there,

	Beside Sakura, Therm and Mopec, does anybody has anyother manufacturers for histology equipments for recommendation (embedding
centers, grossing tables, cassette printers, slide stainers, etc)	

Tks a lot

 

Ms. Kátia Catunda
Produção
+55 12 3203-0612 (direto)
+55 12 3203-0633 (PABX)
www.cipax.com.br
katia.catunda <@t> cipax.com.br





------------------------------

Message: 3
Date: Tue, 13 Nov 2007 12:31:34 -0600
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: staining of negative controls with IHC
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<C28BAF593DC3314E9C0F3A50191C2E7807A3E6AA <@t> EXCHKC03.stowers-institute.org>
	
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MaryAnn Dixon wrote:
"We are currently retrieving animal tissue in a pressure cooker with a program of 125 degrees for 30 secs.  This protocol was taken from biocare but I'm sure this is for human tissue. Most of the tissue is retrieved with Biocare's Reveal (pH 6.0).  We are receiving some background staining of our negative controls whereas if we did not retrieve them, there is none.  We were staining for KI-67 which is a nuclear stain. I am using a tonsil for the control. The negative control showed some sporatic cytoplasmic staining on the Universal negative control from biocare as well as deionized water. Since then we have taken the temperature down to 115 degrees and increased the time to 25 minutes.  It seems to have decreased the cytoplasmic staining but there is still some lingering.  Anyone out there have any ideas??? I'm sure there is a better protocol for retrieving animal tissues. It has to be a time and temperature thing!"

MaryAnn, you don't mention what type of animal you are staining, and what your IHC protocol looks like.

I would never use a universal negative control  when doing immunostaining on animals. Use only the Igs from the species the primary antibody was made in. If you are using a monoclonal mouse KI-67, you would need to use non-immune mouse IgGs of the same isotype of your primary antibody. If your antibody is a rabbit polyclonal or monoclonal, you'd need to use non-immune rabbit IgG. It's also important that you match the Ig concentration fo the non-immune (negative) control to the Ig concentration of your primary antibody. If you are using pre-diluted antibodies, you may be using different Ig concentrations.

Try an additional run to see if your detection system is causing the staining. Run a tonsil, and do the pressure cooker retrieval as usual, however and omit the primary antibody, using only diluent or buffer in the incubation. Then continue your IHC protocol as usual. If there is still some background/non-specific staining, it's due to your detection system (kit?) and not to the antibody or negative control reagent.

Additionally, I never use a universal reagent for detection. Making sure your antibody reactions are appropriate are difficult enough without throwing in additional animal species you don't need.

If you still have specific questions, don't hesitate to contact me off-list and I'll be happy to take a look at your protocol and make some recommendations.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110



------------------------------

Message: 4
Date: Tue, 13 Nov 2007 12:35:36 -0600
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] processing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<0E394B648E5284478A6CCB78E5AFDA2705635279 <@t> hpes1.HealthPartners.int>
Content-Type: text/plain;	charset="us-ascii"

Now that we can no longer use alcoholic fixatives on breast tissue, is there anyone out there that is putting a slight temperature on the reagents on the processor to enhance fixation and dehydration?  I have thought of using stronger alcohol grades and an extra xylene, which would help in the dehydration of the lipids.  This would be a dedeicated processor for fatty tissue types only.  What is everyone's thoughts and thanks for all input!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care ________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.


------------------------------

Message: 5
Date: Tue, 13 Nov 2007 10:42:51 -0800
From: "Tarango, Mark" <mtarango <@t> nvcancer.org>
Subject: RE: [Histonet] processing
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<5AEC610C1CE02945BD63A395BA763EDE011B6F9C <@t> NVCIEXCH02.NVCI.org>
Content-Type: text/plain; charset=us-ascii

Can you use 20% formalin on breast tissue?


Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
  
  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Tuesday, November 13, 2007 10:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing

Now that we can no longer use alcoholic fixatives on breast tissue, is there anyone out there that is putting a slight temperature on the reagents on the processor to enhance fixation and dehydration?  I have thought of using stronger alcohol grades and an extra xylene, which would help in the dehydration of the lipids.  This would be a dedeicated processor for fatty tissue types only.  What is everyone's thoughts and thanks for all input!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care ________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________
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"EMF <nvcancer.org>" made the following annotations.
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Message: 6
Date: Tue, 13 Nov 2007 14:03:15 -0500
From: "Tom McNemar" <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] processing
To: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<51D5D78FBEDAEA4FBCCD9A9D44211DC528F4F4 <@t> lmhsmail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

Why can't you use alcoholic fixatives?


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Webb, Dorothy L
Sent: Tuesday, November 13, 2007 1:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing


Now that we can no longer use alcoholic fixatives on breast tissue, is there anyone out there that is putting a slight temperature on the reagents on the processor to enhance fixation and dehydration?  I have thought of using stronger alcohol grades and an extra xylene, which would help in the dehydration of the lipids.  This would be a dedeicated processor for fatty tissue types only.  What is everyone's thoughts and thanks for all input!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care ________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Tue, 13 Nov 2007 08:13:28 -0800
From: "Thomas Jasper" <tjasper <@t> copc.net>
Subject: [Histonet] Histonet down?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<90354A475B420441B2A0396E5008D4965E1FF9 <@t> copc-sbs.COPC.local>
Content-Type: text/plain; charset="us-ascii"

Anyone having trouble with the Histonet?  Haven't seen any postings for a while. T. Jasper


------------------------------

Message: 8
Date: Tue, 13 Nov 2007 12:40:13 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Histology equipments
To: "Ms. Kátia Cristina Catunda" <katia.catunda <@t> cipax.com.br>,
	'Histology Network' <histonet <@t> Pathology.swmed.edu>
Message-ID: <9152.47378.qm <@t> web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

There are many, but Sakura is the best.
  René J.

"Ms. Kátia Cristina Catunda" <katia.catunda <@t> cipax.com.br> wrote:
  Hi there,

Beside Sakura, Therm and Mopec, does anybody has anyother manufacturers for histology equipments for recommendation (embedding centers, grossing tables, cassette printers, slide stainers, etc) 

Tks a lot

 

Ms. Kátia Catunda
Produção
+55 12 3203-0612 (direto)
+55 12 3203-0633 (PABX)
www.cipax.com.br
katia.catunda <@t> cipax.com.br



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Message: 9
Date: Wed, 14 Nov 2007 06:58:44 +1000
From: "Barry Madigan" <barry_m <@t> ozemail.com.au>
Subject: RE: [Histonet] Antibody cocktails
To: "'Christine I. Braaten'" <CBraaten <@t> Cheshire-Med.COM>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c82638$012fe800$038fb800$@com.au>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Christine,

We make up our own antibody cocktail of 34ßE12 (DAKO dilution 1:150) and P63 (DAKO 1:400) and stain the section with this cocktail with a Polymer peroxidise detection system (DAB).(Heat retrieval in a high pH retrieval solution for 20 min at 100 C.

The stained slides are washed and darkened using a copper sulphate solution. Placed in a buffer wash and then stained for the second time with P504S
(AMACR/Racemase) at a dilution of 1:150 with a Polymer Alkaline Phosphatase detection system (Fast Red). We do this all on the BondMax Immunostainer. We have been doing this for over 6 months now and did it this way because we already had all the concentrated antibodies. Proper fixation is vital.

Hope this helps.

Regards
Barry 
B.Madigan
Pathology Queensland
Royal Brisbane Campus
Australia







------------------------------

Message: 10
Date: Tue, 13 Nov 2007 16:32:03 -0500
From: Eva C Andersson <eca9 <@t> georgetown.edu>
Subject: [Histonet] microtomy technical question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <163fae165e00.165e00163fae <@t> imap.georgetown.edu>
Content-Type: text/plain; charset=us-ascii

Hello everyone,
I have some questions regarding microtomy. 
We place our blocks face down on ice. The problem is that some tissues take a very long time before they are ready to be cut(some more than 6 hours). For some tissues like kidney samples (mouse tissue) we have been using glycerol on the ice. This does seem to cut down on the time needed. My question is which other tissues can I use this technique on? Do you have any other suggestions for how to get the tissue hydrated for cutting? Thank you for your help, Eva Permaul Georgetown University



------------------------------

Message: 11
Date: Tue, 13 Nov 2007 13:39:58 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] microtomy technical question
To: Eva C Andersson <eca9 <@t> georgetown.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <975592.48450.qm <@t> web61220.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Eva:
  This type of problem (requiring ice, glycerol, and others) to allow sectioning usually stem from either a fixing or processing defficiency. I suggest you to check your fixing times and processing protocols or you will keep having this type of difficulties.
  Tissues (any type) properly infiltrated (after adequate fixation/processing) do not need anything but a light cooling to allow ribbons to be taken.
  René J.

Eva C Andersson <eca9 <@t> georgetown.edu> wrote:
  Hello everyone,
I have some questions regarding microtomy. 
We place our blocks face down on ice. The problem is that some tissues take a very long time before they are ready to be cut(some more than 6 hours). For some tissues like kidney samples (mouse tissue) we have been using glycerol on the ice. This does seem to cut down on the time needed. My question is which other tissues can I use this technique on? Do you have any other suggestions for how to get the tissue hydrated for cutting? Thank you for your help, Eva Permaul Georgetown University

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Message: 12
Date: Tue, 13 Nov 2007 14:57:37 -0700
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] It may be more than just a microtomy technical
	question
To: "Eva C Andersson" <eca9 <@t> georgetown.edu>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c82640$33f907a0$6501a8c0 <@t> DHXTS541>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Eva,

It sounds as though you are processing your tissues too long, and too much 
water is being removed (over dehydration.)   In general, a properly 
processed mouse tissue after a short schedule will need a bit of a water 
soak, but not for 6 hours.  Another trick is try room temperature or even 
warm water for a few minutes, then go to ice water, and be careful to NOT 
cut away what you have soaked - be careful to reapproach the blade in order 
to cut the sections.  Some people have suggested floating blocks face down 
on a waterbath for a very short time.

if you post your processing schedule, Histonetters can help solve some of 
this problem.

Gayle Callis
HT,HTL,MT(ASCP)



----- Original Message ----- 
From: "Eva C Andersson" <eca9 <@t> georgetown.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 13, 2007 2:32 PM
Subject: [Histonet] microtomy technical question


> Hello everyone,
> I have some questions regarding microtomy.
> We place our blocks face down on ice. The problem is that some tissues
> take a very long time before they are ready to be cut(some more than 6 
> hours). For some tissues like kidney samples (mouse tissue) we have been 
> using glycerol on the ice. This does seem to cut down on the time needed. 
> My question is which other tissues can I use this technique on? Do you 
> have any other suggestions for how to get the tissue hydrated for cutting?
> Thank you for your help,
> Eva Permaul
> Georgetown University
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 13
Date: Tue, 13 Nov 2007 15:00:06 -0800 (PST)
From: Schaundra Walton <schaundrawalton <@t> yahoo.com>
Subject: [Histonet] Giemsa Stain
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <625759.21602.qm <@t> web58904.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We've been using a Giemsa stain for our H. pylori cases without a control tissue.  It has come to my attention that this may not be good practice.  I was under the impression that the Giemsa stain had an internal control.  Is anyone else using a this stain for H. pylori?  Are you running a tissue control with it?

       
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Message: 14
Date: Wed, 14 Nov 2007 05:48:41 -0500
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> CDC.GOV>
Subject: RE: [Histonet] automated paraffin embedding systems
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,	"Lesley Bechtold"
	<lesley.bechtold <@t> jax.org>,	"Histology Network"
	<histonet <@t> Pathology.swmed.edu>
Message-ID:
	<34BB307EFC9A65429BBB49E330675F72045E21F1 <@t> LTA3VS003.ees.hhs.gov>
Content-Type: text/plain; charset=iso-8859-1

Slight correction.  The Xpress microwave tissue processor does not require a special type of cassette.  The AutoTEC does, and it can be used on the Xpress or in a traditional processor.  But the Xpress can use the same type of plastic cassette used in any traditional processor.


Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 13, 2007 12:04 PM
To: Lesley Bechtold; Histology Network
Subject: Re: [Histonet] automated paraffin embedding systems

This embedding instrument is manufactured by Sakura. It is not that the instrument orients the tissue, that is done when the tissues are placed in the cassette and held in place by a plastic frame that will be sectioned along with the tissue when the block is prepared. To function ideally it requires a special type of cassette and cassette holder (that is used alsao by the Sakura Xpress microwave tissue processor).
  René J.

Lesley Bechtold <lesley.bechtold <@t> jax.org> wrote:
  Hi, 

I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?

Thank you!

Lesley


Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322


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Message: 15
Date: Wed, 14 Nov 2007 05:50:24 -0500
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> CDC.GOV>
Subject: RE: [Histonet] automated paraffin embedding systems
To: "Mike Pence" <mpence <@t> grhs.net>, "Rene J Buesa"
	<rjbuesa <@t> yahoo.com>,	"Lesley Bechtold" <lesley.bechtold <@t> jax.org>,
	"Histology Network" <histonet <@t> Pathology.swmed.edu>
Message-ID:
	<34BB307EFC9A65429BBB49E330675F72045E21F2 <@t> LTA3VS003.ees.hhs.gov>
Content-Type: text/plain; charset=iso-8859-1

I use both machines.  You can use the AutoTEC cassette on any type of processor (including the Xpress) and you do not have to use the AutoTEC and the Xpress both.  They can be used independently. 


Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday, November 13, 2007 12:10 PM
To: Rene J Buesa; Lesley Bechtold; Histology Network
Subject: RE: [Histonet] automated paraffin embedding systems

Is anyone out there using this function of the Sakura Xpress? Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, November 13, 2007 11:04 AM
To: Lesley Bechtold; Histology Network
Subject: Re: [Histonet] automated paraffin embedding systems


This embedding instrument is manufactured by Sakura. It is not that the instrument orients the tissue, that is done when the tissues are placed in the cassette and held in place by a plastic frame that will be sectioned along with the tissue when the block is prepared. To function ideally it requires a special type of cassette and cassette holder (that is used alsao by the Sakura Xpress microwave tissue processor).
  René J.

Lesley Bechtold <lesley.bechtold <@t> jax.org> wrote:
  Hi, 

I've heard rumors that there are systems for doing automated paraffin embedding AND that they orient tissue correctly. Could someone (vendor, customer, anyone) please point me in the right direction?

Thank you!

Lesley


Lesley S. Bechtold
Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322


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Message: 16
Date: Wed, 14 Nov 2007 05:01:09 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Giemsa Stain
To: Schaundra Walton <schaundrawalton <@t> yahoo.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <387310.3350.qm <@t> web61217.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Giemsa, as any other stain, is just that, a stain, and stains do not (cannot) have "internal controls".
  As with any other HC procedure you have to use a positive control along with your tissue.
  To completely demonstrate that your Giemsa procedure worked as expected, you coul use a blood smear or an appendix section as controls. That will depend on your pathologist's whims.
  René J.

Schaundra Walton <schaundrawalton <@t> yahoo.com> wrote:
  We've been using a Giemsa stain for our H. pylori cases without a control tissue. It has come to my attention that this may not be good practice. I was under the impression that the Giemsa stain had an internal control. Is anyone else using a this stain for H. pylori? Are you running a tissue control with it?


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Message: 17
Date: Wed, 14 Nov 2007 13:22:36 -0000
From: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Giemsa Stain
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,	"Schaundra Walton"
	<schaundrawalton <@t> yahoo.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5C0BED61F529364E86309CADEA63FEF2F3AE79 <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain;	charset="iso-8859-1"

Sorry Rene, on this occasion I can't agree.
Giemsa is not on this occasion being used to bring out granules as it would in it's normal use as a blood/marrow stain. It is just used as a simple quick stain, which will pick up Helicobacter inter alia. If the tissue is blue, it has worked, at least to the extent it needs to have done for this purpose.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 14 November 2007 13:01
To: Schaundra Walton; Histonet
Subject: Re: [Histonet] Giemsa Stain

Giemsa, as any other stain, is just that, a stain, and stains do not (cannot) have "internal controls".
  As with any other HC procedure you have to use a positive control along with your tissue.
  To completely demonstrate that your Giemsa procedure worked as expected, you coul use a blood smear or an appendix section as controls. That will depend on your pathologist's whims.
  René J.

Schaundra Walton <schaundrawalton <@t> yahoo.com> wrote:
  We've been using a Giemsa stain for our H. pylori cases without a control tissue. It has come to my attention that this may not be good practice. I was under the impression that the Giemsa stain had an internal control. Is anyone else using a this stain for H. pylori? Are you running a tissue control with it?


---------------------------------
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------------------------------

Message: 18
Date: Wed, 14 Nov 2007 05:41:09 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] Giemsa Stain
To: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>, 	Schaundra Walton
	<schaundrawalton <@t> yahoo.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <246072.35484.qm <@t> web61218.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I was referring at the fact that IF some coloration took place (of any kind) the procedure worked, and there was a staining. I was not referring to stain specificity.
  René J.

"Marshall Terry Dr, Consultant Histopathologist" <Terry.Marshall <@t> rothgen.nhs.uk> wrote:
  Sorry Rene, on this occasion I can't agree.
Giemsa is not on this occasion being used to bring out granules as it would in it's normal use as a blood/marrow stain. It is just used as a simple quick stain, which will pick up Helicobacter inter alia. If the tissue is blue, it has worked, at least to the extent it needs to have done for this purpose.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: 14 November 2007 13:01
To: Schaundra Walton; Histonet
Subject: Re: [Histonet] Giemsa Stain

Giemsa, as any other stain, is just that, a stain, and stains do not (cannot) have "internal controls". As with any other HC procedure you have to use a positive control along with your tissue. To completely demonstrate that your Giemsa procedure worked as expected, you coul use a blood smear or an appendix section as controls. That will depend on your pathologist's whims. René J.

Schaundra Walton wrote:
We've been using a Giemsa stain for our H. pylori cases without a control tissue. It has come to my attention that this may not be good practice. I was under the impression that the Giemsa stain had an internal control. Is anyone else using a this stain for H. pylori? Are you running a tissue control with it?


---------------------------------
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Message: 19
Date: Wed, 14 Nov 2007 13:42:27 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Giemsa Stain
To: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>, 	"Rene J Buesa" <rjbuesa <@t> yahoo.com>,
	"Schaundra Walton" <schaundrawalton <@t> yahoo.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<86ADE4EB583CE64799A9924684A0FBBF0222F171 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

"If the tissue is blue, it has worked, at least to the extent it needs to have done for this purpose." Terry

If it's not blue then it hasn't worked or the organism isn't there!


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places. --Author Unknown 

This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation
 




------------------------------

Message: 20
Date: Wed, 14 Nov 2007 13:43:25 +0000
From: koellingr <@t> comcast.net
Subject: Re: [Histonet] Giemsa Stain
To: Rene J Buesa <rjbuesa <@t> yahoo.com>,	Schaundra Walton
	<schaundrawalton <@t> yahoo.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<111420071343.9538.473AFB7D00086C1A0000254222058863609D09020704040A0105 <@t> comcast.net>
	
Content-Type: text/plain

Rene,
Are you saying a histochemical stain for an organism (Helicobactor, AFB, spirochetes, etc) must have a positive control with which I'd agree?  But are you saying that for "any" HC stain like elastic, trichrome, PAS, alcian blue, etc that internal controls don't (cannot) even exist? Thanks,

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message -------------- 
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

> Giemsa, as any other stain, is just that, a stain, and stains do not 
> (cannot)
> have "internal controls". 
> As with any other HC procedure you have to use a positive control along with 
> your tissue. 
> To completely demonstrate that your Giemsa procedure worked as expected, you 
> coul use a blood smear or an appendix section as controls. That will depend on 
> your pathologist's whims. 
> Ren$B!&(BJ. 
> 
> Schaundra Walton wrote:
> We've been using a Giemsa stain for our H. pylori cases without a control 
> tissue. It has come to my attention that this may not be good practice. I was 
> under the impression that the Giemsa stain had an internal control. Is anyone 
> else using a this stain for H. pylori? Are you running a tissue control with it? 
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. 
> _______________________________________________ 
> Histonet mailing list 
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile. Try it now. 
> _______________________________________________ 
> Histonet mailing list 
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 21
Date: Wed, 14 Nov 2007 13:50:33 -0000
From: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Giemsa Stain
To: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>,	"Rene J
	Buesa" <rjbuesa <@t> yahoo.com>,	"Schaundra Walton"
	<schaundrawalton <@t> yahoo.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5C0BED61F529364E86309CADEA63FEF2F3AE7A <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

No.
The tissue can still be blue even if the organism isn't there.

BTW, has anyone ever seen a Giemsa that doesn't stain? I can't really imagine it.

Terry 

-----Original Message-----
From: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> waht.swest.nhs.uk] 
Sent: 14 November 2007 13:42
To: Marshall Terry Dr, Consultant Histopathologist; Rene J Buesa; Schaundra Walton; Histonet
Subject: RE: [Histonet] Giemsa Stain

"If the tissue is blue, it has worked, at least to the extent it needs to have done for this purpose." Terry

If it's not blue then it hasn't worked or the organism isn't there!


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places. --Author Unknown 

This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation
 





------------------------------

Message: 22
Date: Wed, 14 Nov 2007 13:53:15 -0000
From: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Giemsa Stain
To: <koellingr <@t> comcast.net>, "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
	"Schaundra Walton" <schaundrawalton <@t> yahoo.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5C0BED61F529364E86309CADEA63FEF2F3AE7B <@t> TRFT-EX01.xRothGen.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

So we are singing from the same hymn sheet....
Stains can't have internal controls.
Tissues can, in relation to some stains.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: 14 November 2007 13:43
To: Rene J Buesa; Schaundra Walton; Histonet
Subject: Re: [Histonet] Giemsa Stain

Rene,
Are you saying a histochemical stain for an organism (Helicobactor, AFB, spirochetes, etc) must have a positive control with which I'd agree? But are you saying that for "any" HC stain like elastic, trichrome, PAS, alcian blue, etc that internal controls don't (cannot) even exist? Thanks,

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message --------------
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

> Giemsa, as any other stain, is just that, a stain, and stains do not
> (cannot) have "internal controls".
> As with any other HC procedure you have to use a positive control 
> along with your tissue.
> To completely demonstrate that your Giemsa procedure worked as 
> expected, you coul use a blood smear or an appendix section as 
> controls. That will depend on your pathologist's whims.
> Ren$B!&(BJ. 
> 
> Schaundra Walton wrote:
> We've been using a Giemsa stain for our H. pylori cases without a 
> control tissue. It has come to my attention that this may not be good 
> practice. I was under the impression that the Giemsa stain had an 
> internal control. Is anyone else using a this stain for H. pylori? Are
you running a tissue control with it?
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile.
Try it now. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile.
Try it now. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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------------------------------

Message: 23
Date: Wed, 14 Nov 2007 13:54:07 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] Giemsa Stain
To: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>, 	"Rene J Buesa" <rjbuesa <@t> yahoo.com>,
	"Schaundra Walton" <schaundrawalton <@t> yahoo.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<86ADE4EB583CE64799A9924684A0FBBF0222F172 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain;	charset="us-ascii"

"No.
The tissue can still be blue even if the organism isn't there." Terry

I know what you mean; I get days like that too.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places. --Author Unknown 

This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation
 




------------------------------

Message: 24
Date: Wed, 14 Nov 2007 05:57:03 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: RE: [Histonet] Giemsa Stain
To: "Marshall Terry Dr,	Consultant Histopathologist"
	<Terry.Marshall <@t> rothgen.nhs.uk>, 	koellingr <@t> comcast.net, Schaundra
	Walton <schaundrawalton <@t> yahoo.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <588286.70996.qm <@t> web61222.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Amen!
René J.

"Marshall Terry Dr, Consultant Histopathologist" <Terry.Marshall <@t> rothgen.nhs.uk> wrote:  So we are singing from the same hymn sheet.... Stains can't have internal controls. Tissues can, in relation to some stains.

Terry 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: 14 November 2007 13:43
To: Rene J Buesa; Schaundra Walton; Histonet
Subject: Re: [Histonet] Giemsa Stain

Rene,
Are you saying a histochemical stain for an organism (Helicobactor, AFB, spirochetes, etc) must have a positive control with which I'd agree? But are you saying that for "any" HC stain like elastic, trichrome, PAS, alcian blue, etc that internal controls don't (cannot) even exist? Thanks,

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message --------------
From: Rene J Buesa 

> Giemsa, as any other stain, is just that, a stain, and stains do not
> (cannot) have "internal controls".
> As with any other HC procedure you have to use a positive control 
> along with your tissue.
> To completely demonstrate that your Giemsa procedure worked as 
> expected, you coul use a blood smear or an appendix section as 
> controls. That will depend on your pathologist's whims.
> Ren$B!&(BJ. 
> 
> Schaundra Walton wrote:
> We've been using a Giemsa stain for our H. pylori cases without a 
> control tissue. It has come to my attention that this may not be good 
> practice. I was under the impression that the Giemsa stain had an 
> internal control. Is anyone else using a this stain for H. pylori? Are
you running a tissue control with it?
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile.
Try it now. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> ---------------------------------
> Be a better sports nut! Let your teams follow you with Yahoo Mobile.
Try it now. 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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---------------------------------
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------------------------------

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End of Histonet Digest, Vol 48, Issue 18
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