[Histonet] Re: Giemsa stain

Robert Richmond RSRICHMOND <@t> aol.com
Wed Nov 14 08:53:57 CST 2007

The Giemsa stain is widely used as a stain for Helicobacter pylori in
gastric biopsy material. It's actually simpler to use a solution of
toluidine blue and related dyes in a neutral buffer (such as Diff-Quik
II and its many generic equivalents). There is adequate literature to
support this technique.

It's never good practice to stain for an organism without an
appropriate control slide, though in this particular case I don't
really think it's necessary from a technical viewpoint - the adjacent
tissue provides an adequate control.

In the USA, some regulatory agencies (this is a somewhat controversial
point) require mention of a control in the pathologist's report. My
usual microscopic note reads "a bacterial stain (generic equivalent of
Diff-Quik II, with a positive control slide) shows nothing to suggest

If the control slide doesn't contain any bacteria (frequently the
case, with histotechs who never look at a slide), I say "suitable"
rather than "positive".

The control should be a gastric biopsy specimen positive for
Helicobacter. The pathologist should inform the histotechs when a
suitable case to use as a control comes by. Of course, if you get a
gastrectomy specimen done for ulcer disease (shouldn't happen, but it
still does occasionally) then you're set for control sections for

Slides often need to be examined with an oil immersion lens. I do this
with all positive specimens, and with apparent negatives when chronic
active gastritis (neutrophils infiltrating between the gastric pits)
is present.

I don't think immunohistochemistry is more sensitive, but reading the
slides is an awful lot faster - important if you've got 10 gastric
biopsies on your desk. I have no experience with the silver techniques
occasionally used.

Bob Richmond
Samurai Pathologist
Knoxville TN

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