[Histonet] Giemsa Stain

[koellingr <@t> comcast.net]

Rene,
Are you saying a histochemical stain for an organism (Helicobactor, AFB, spirochetes, etc) must have a positive control with which I'd agree?  But are you saying that for "any" HC stain like elastic, trichrome, PAS, alcian blue, etc that internal controls don't (cannot) even exist?
Thanks,

Ray Koelling
PhenoPath Labs
Seattle, WA

-------------- Original message -------------- 
From: Rene J Buesa <rjbuesa <@t> yahoo.com> 

> Giemsa, as any other stain, is just that, a stain, and stains do not (cannot) 
> have "internal controls". 
> As with any other HC procedure you have to use a positive control along with 
> your tissue. 
> To completely demonstrate that your Giemsa procedure worked as expected, you 
> coul use a blood smear or an appendix section as controls. That will depend on 
> your pathologist's whims. 
> Ren$B!&(BJ. 
> 
> Schaundra Walton wrote: 
> We've been using a Giemsa stain for our H. pylori cases without a control 
> tissue. It has come to my attention that this may not be good practice. I was 
> under the impression that the Giemsa stain had an internal control. Is anyone 
> else using a this stain for H. pylori? Are you running a tissue control with it? 
> 
> 
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