[Histonet] It may be more than just a microtomy technical question

[Gayle Callis]

Eva,

It sounds as though you are processing your tissues too long, and too much 
water is being removed (over dehydration.)   In general, a properly 
processed mouse tissue after a short schedule will need a bit of a water 
soak, but not for 6 hours.  Another trick is try room temperature or even 
warm water for a few minutes, then go to ice water, and be careful to NOT 
cut away what you have soaked - be careful to reapproach the blade in order 
to cut the sections.  Some people have suggested floating blocks face down 
on a waterbath for a very short time.

if you post your processing schedule, Histonetters can help solve some of 
this problem.

Gayle Callis
HT,HTL,MT(ASCP)



----- Original Message ----- 
From: "Eva C Andersson" <eca9 <@t> georgetown.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 13, 2007 2:32 PM
Subject: [Histonet] microtomy technical question


> Hello everyone,
> I have some questions regarding microtomy.
> We place our blocks face down on ice. The problem is that some tissues 
> take a very long time before they are ready to be cut(some more than 6 
> hours). For some tissues like kidney samples (mouse tissue) we have been 
> using glycerol on the ice. This does seem to cut down on the time needed. 
> My question is which other tissues can I use this technique on? Do you 
> have any other suggestions for how to get the tissue hydrated for cutting?
> Thank you for your help,
> Eva Permaul
> Georgetown University
>
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