[Histonet] processing

[Tom McNemar]

Why can't you use alcoholic fixatives?


Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Webb,
Dorothy L
Sent: Tuesday, November 13, 2007 1:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing


Now that we can no longer use alcoholic fixatives on breast tissue, is
there anyone out there that is putting a slight temperature on the
reagents on the processor to enhance fixation and dehydration?  I have
thought of using stronger alcohol grades and an extra xylene, which
would help in the dehydration of the lipids.  This would be a dedeicated
processor for fatty tissue types only.  What is everyone's thoughts and
thanks for all input!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care
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