[Histonet] deplasticizing PMMA sections for staining

[Michelle McDonald]

We actually use Acetone to deplasticise MMA sections. Only takes 2
changes for 10 mins each.

Michelle

Dr Michelle McDonald B.MedSci, PhD
 
Research Officer
Orthopaedic Research Dept.
The Children's Hospital Westmead.
Westmead NSW 2145
Australia
 
Ph. +612 9845 1451
Fax. +612 9845 3078
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Monfils,
Paul
Sent: Tuesday, 6 November 2007 6:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] deplasticizing PMMA sections for staining


In my experience, xylene is a rather poor solvent for PMMA.  It works
much faster at 60 degrees, but don't try heating flammable solvents in
your oven unless you are certain it is certified explosion-proof (most
paraffin ovens are not).  Even then, unless you have the oven in or at
least next to a fume hood, you'll get a lot of xylene vapors in the air
as a result of heating it.  I deplasticise PMMA with 2-methoxyethyl
acetate, which only takes a couple of hours at room temperature.  This
has a rather strong though not unpleasant odor, and should be used in
the hood.

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