[Histonet] frozen bat brain

[I-sanna Gibbons]

Thanks for all the suggestions!  Initially I was producing paraffin sections of bat brains but the results were not satisfactory.  I am recording cell structure of the nuclei in the brainstem.  I still have a number of brains fixing in 10% formalin and now want to change to produce frozen sections without compromising cell structure.  Most of you suggested starting off by immersing the brain in 30% sucrose (cryoprotectant) until the tissue sinks.  Does anyone have a detailed procedure (material, duration, temperature) for whole brains that can follow after this step or know of a site to which I can be directed that will provide me with that information?

Thanks a lot
I-sanna

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