[Histonet] frozen bat brain
trinimaican2501 <@t> yahoo.com
Mon Nov 12 09:32:54 CST 2007
Thanks for all the suggestions! Initially I was producing paraffin sections of bat brains but the results were not satisfactory. I am recording cell structure of the nuclei in the brainstem. I still have a number of brains fixing in 10% formalin and now want to change to produce frozen sections without compromising cell structure. Most of you suggested starting off by immersing the brain in 30% sucrose (cryoprotectant) until the tissue sinks. Does anyone have a detailed procedure (material, duration, temperature) for whole brains that can follow after this step or know of a site to which I can be directed that will provide me with that information?
Thanks a lot
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