[Histonet] Clone for Prog. Receptor

[koellingr <@t> comcast.net]

Sally,
You asked about PR but there is a lot of discussion about mouse monoclonals versus rabbit monoclonals in general and as there is a lot of "fact" floating around that is somewhere between questionable to outright fallacy, I thought I'd chime in.  In a former life, we were involved with the company that originated the rabbit monoclonal Ab technology as well as some companies that they licensed their technology to.  Anyone stating that rabbit monoclonals have higher affinities than their cousin mouse monoclonals as a general concept have some real experimental science to do.  For reasons that would take a page or 2, I cannot do so here.  Whether by IHC staining you get "more" staining or "less" staining signal with rabbits versus mouse Mab's, might have nothing what-so-ever to do with their relative affinities to tissue target.  The readers digest version of this is that you need to do "Biacore" type (surface plasmon resonance technology) to determine affinities of antibodies.  As it 
happens, one of the only (first?) articles published I've ever seen doing this to bring some sense to this controversial subject was published in the latest journal of the Journal of Histotechnology.  Their results, I think will jolt a few of the preconceived notions that some have of the utter and absolute superiority of Rabbit Mab's over Mouse Mab's.  A caveat,  this was done by Vision Biosystems themselves.  I think a great piece for a world-wide peer-reviewed journal would be to do their analysis (x5 or 6 antibodies) and with several more experiments and it would be to great service and utility for the entire IHC and general antibody world.  I like Rabbit Ab's, have used and use them.  Some utterly superior to Mouse Mab's for IHC in my hands.  But some are not nearly as good and even terrible, in my hands and this may or may not have one thing to do with affinity.

Raymond Koelling
PhenoPath Laboratories
Seattle, WA

-------------- Original message -------------- 
From: <pruegg <@t> ihctech.net> 

> I do not have the clone, but I use rab monoclonal pr from Lab Vision with 
> good results, I use a labeled polymer detection. 
> Patsy 
> 
> -----Original Message----- 
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard 
> Cartun 
> Sent: Thursday, November 08, 2007 10:40 AM 
> To: Histonet; Drew Sally A. 
> Subject: Re: [Histonet] Clone for Prog. Receptor 
> 
> We have used clone PgR636 (from Dako) for several years now with excellent 
> results. 
> 
> Richard 
> 
> Richard W. Cartun, Ph.D. 
> Director, Immunopathology & Histology 
> Assistant Director, Anatomic Pathology 
> Hartford Hospital 
> 80 Seymour Street 
> Hartford, CT 06102 
> (860) 545-1596 
> (860) 545-0174 Fax 
> 
> >>> "Drew Sally A." 11/08/07 12:07 PM >>> 
> We currently are using the 1A6 clone for our progesterone receptor(PR) 
> IHC. 
> We were wondering how other people felt about rabbit monoclonal PR 
> antibodies. 
> Does anyone have strong preferences or experiences with other PR 
> clones-mouse or rabbit-that 
> they'd like to share? 
> Thank you...! 
> 
> Sally Ann Drew, MT(ASCP) 
> IHC/ISH Laboratory 
> University of Wisconsin Hosp. & Clinics 
> Madison, WI 53792 
> (608)265-6596 
> 
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