[Histonet] frozen brain

pruegg <@t> ihctech.net pruegg <@t> ihctech.net
Sat Nov 10 10:07:32 CST 2007

After fixation I always infiltrate the tissue in 30% sucrose at least
overnight before freezing.  The morphology is very well preserved and frozen
sections are great improved over trying to cut nbf fixed tissue frozen
without sucrose infiltration.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of I-sanna
Sent: Thursday, November 08, 2007 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] frozen brain

Hi all,
Does anyone have a protocol for freezing fixed (10% formalin) brain tissue?
Will cell morphology be compromised to a large extent?

Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list