[Histonet] Hematoxylin to dark on some slides

[Cheri Miller]

Who is cutting them?  I know I can have 2 techs cutting at the same microns,
same tissue and one's sections will always be thicker regardless of which
microtome they are cutting on. I have come to the conclusion its all in the
technique of each individual tech. Cheri

Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Bledsoe
Sent: Friday, November 09, 2007 6:37 AM
To: Kemlo Rogerson; Dawn Bugge; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Hematoxylin to dark on some slides

Or the slice thickness



At 8:03 AM +0000 11/9/07, Kemlo Rogerson wrote:
>You mean you stain at the same time and those cut at night are staining
>darker with haematoxylin than those cut during the day. If you are
>staining those cut at night, also at night, maybe you can't see the
>clock?
>
>Kemlo Rogerson
>Pathology Manager
>DD   01934 647057 or extension 3311
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