[Histonet] Dendritic cells in mouse FFPE tissue

[Tarango, Mark]

What kind of dendritic cells?  I have a research antibody that I SWEAR
is marking the nuclei of dendritic cells.  The researcher who made the
antibody claims they're stem cells though.  What do I know?

Those langerhans kinds of dendritic cells should be CD1a positive and
BIOCARE has an antibody that works on paraffin.  You might try CD11c
too.

For plasmacytoid dendritic cells or follicular center/germinal center
dendritic cells I don't know of any single marker that is great and
won't mark anything aside from the dendritic cells.  You don't want any
cross-reactivity with macrophages, so I guess CD68 ain't an option...

Hmmm....

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Milne,
Katy
Sent: Thursday, November 08, 2007 1:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dendritic cells in mouse FFPE tissue

Ok, I know I'm on another one of my wild goose chases again but is
anyone successfully working with an antibody that detects dendritic
cells in mouse FFPE tissue (no, frozen is not an option at this point)?
It needs to be something specific for dendritic cells, i.e. no
cross-reactivity with macrophages etc.

Thanks,
Katy Milne
Deeley Research Centre

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


"EMF <nvcancer.org>" made the following annotations.
------------------------------------------------------------------------------
CONFIDENTIALITY NOTICE: This e-mail message, including any
attachments, is for the sole use of the intended 
recipient(s) and may contain confidential, proprietary, 
and/or privileged information protected by law. If you are 
not the intended recipient, you may not use, copy, or 
distribute this e-mail message or its attachments. If you 
believe you have received this e-mail message in error, 
please contact the sender by reply e-mail and destroy all 
copies of the original message
==============================================================================