[Histonet] B5 substitute

[Rene J Buesa]

Amen! Only make absolutely sure that the pH of your NBF is neutral (7).
René J.

"Tarango, Mark" <mtarango <@t> nvcancer.org> wrote:  Why not just use 10% NBF? It's great if you allow enough time for
fixation. Make sure your bone marrows are fixed well BEFORE
decalcifying and you should have no problems.


Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
Mauger
Sent: Thursday, November 08, 2007 8:51 AM
To: gvdobbin <@t> ihis.org; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] B5 substitute

Hi ,

What are you using to replace B5 fixative with mercury? I want the best
for hematopoetic tissues, and for immunostains.

Thanks,

Jo Mauger

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