[Histonet] viability stain for 450 micron brain slices

Tarango, Mark mtarango <@t> nvcancer.org
Wed Nov 7 18:19:58 CST 2007

The first thing that comes to mind is Trypan blue, but that would be for
regular light microscopy.  If you want to do florescent you might try
7AAD or propidium idodide.  An ethidium bromide / acridine orange combo
might be good too.  

I'm not sure about fixing the staining.  

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sent: Wednesday, November 07, 2007 6:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] viability stain for 450 micron brain slices

I am looking for a staining technique to test the viability of 
slices of the rat brain after some force-displacement tests on the 
tissue. As I am an engineer and no chemist I do not know what kind 
of stain would be appropriate. We keep the slices from the 
cerebral cortex and the corpus callosumalive alive in a tissue 
chamber with ACSF that is bubbled with 95% O2/ 5% CO2. The slices 
have a thickness of 450 micron and there is a fluorescent confocal 
microscope available. As this microscope is away from the testing 
site and we do not have the equipment that can keep the tissue 
alive during imaging, it would be very convenient to fix the 
tissue after staining. Does anyone of you have experience with a 
staining technique that is suitable for my experiment?

Best, Kirsten Henken

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