[Histonet] Rocking/shaking slides
TillRenee <@t> uams.edu
Wed Nov 7 11:08:12 CST 2007
Does anyone rock or shake their slides during incubation steps? I recently
had a product e-mail given to me for a slide container intended for this use.
The theory being the same as used during western blots; that it would reduce
the antibody concentration needed for good staining and that the antibody
solution would be reusable.
I have protocols that call for washing steps on a shaker, but I had never
thought of how it might affect the actual antibody incubation nor come across
a protocol that calls for it.
Renee' Till, HT (ASCP)
Arkansas Children's Nutrition Center
1212 Marshall St./N2021
Little Rock, AR 72202
Office Fax (501)364-3161
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
More information about the Histonet