[Histonet] Rocking/shaking slides

[Till, Renee]

Does anyone rock or shake their slides during incubation steps? I recently
had a product e-mail given to me for a slide container intended for this use.
The theory being the same as used during western blots; that it would reduce
the antibody concentration needed for good staining and that the antibody
solution would be reusable. 

I have protocols that call for washing steps on a shaker, but I had never
thought of how it might affect the actual antibody incubation nor come across
a protocol that calls for it. 

 

Renee' Till, HT (ASCP)

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72202

Lab (501)364-8504

Office Fax (501)364-3161

 


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