[Histonet] RE: eosinophil peroxidase

[Melissa Gonzalez]

Hi Michelle, 
I have also only seen one publication for EPO IHC but it was also on frozens with mouse tissue. Have you tried MBP, ECP (via EG1 & EG2), EDN, or eotaxin on human FFPE's with any success?

Melissa

Melissa A. González Edick
R&D, Cell Genesys Inc.
500 Forbes Blvd
South San Francisco, CA 94080
p(650) 266-3168
f (650) 266-3080

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Message: 2
Date: Tue, 6 Nov 2007 08:57:25 -0600
From: "Griffin-Reyes, Michelle A" <michelle-griffin-reyes <@t> uiowa.edu>
Subject: [Histonet] eosinophil peroxidase (EPO)
To: <histonet <@t> lists.utsouthwestern.edu>

Does anyone have any experience using eosinophil peroxidase (EPO) to
stain FFPE tissues (I am doing other staining methods as well)? I have
searched and can find EPO usage for western blot and flow and one
publication using frozen tissues but nothing for FFPE tissues. Any
suggestions would be greatly appreciated. Thanks in advance!!

Michelle A. Griffin-Reyes
Comparative Pathology Laboratory
University of Iowa Hospitals and Clinics
319-384-4620