[Histonet] how to keep thick sections on the slides after deparaffin

[Kemlo Rogerson]

Hi,
 
  I met a big problem in keeping thick sections on the slides after
deparaffin recently. I cut a 70um-thick mouse retina section on
superfrost plus Gold slides, leaving it dry for 2days. Before
deparaffin, I put the slide in the 65C oven for 30 min to let it melt,
then I did the general deparaffin procedure. Unfortunately, the section
fell off from the slide. Does anyone have any experience on deparaffin
thick sections? Any suggestions?
 
  Thank you,

You could use bovine albumin to coat the slides (I think 1% but I'm not
sure) then dry at 37 degrees for those two days then into the oven at 60
degrees (ish). Think that sort of sets the section onto the slide but
you need to get the concentration correct as the protein could stain
with some techniques if its too concentrated.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
No snowflake in an avalanche ever feels responsible. --George Burns 

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