[Histonet] how to keep thick sections on the slides after
Kemlo.Rogerson <@t> waht.swest.nhs.uk
Tue Nov 6 02:24:24 CST 2007
I met a big problem in keeping thick sections on the slides after
deparaffin recently. I cut a 70um-thick mouse retina section on
superfrost plus Gold slides, leaving it dry for 2days. Before
deparaffin, I put the slide in the 65C oven for 30 min to let it melt,
then I did the general deparaffin procedure. Unfortunately, the section
fell off from the slide. Does anyone have any experience on deparaffin
thick sections? Any suggestions?
You could use bovine albumin to coat the slides (I think 1% but I'm not
sure) then dry at 37 degrees for those two days then into the oven at 60
degrees (ish). Think that sort of sets the section onto the slide but
you need to get the concentration correct as the protein could stain
with some techniques if its too concentrated.
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