[Histonet] Feline retina whole mounts - tissue loss

Amy Porter portera <@t> msu.edu
Fri Nov 2 12:20:55 CDT 2007

Help....I have a researcher who for years has used the same sample preparation by placing whole mount feline retinas on 1% Gelatin subbed slides and then vapor (curing) fixing with 37%-40% for 48 hours.  They are suddenly having problems with tissue falling off of their slides.  Does anyone out there have a suggestion or a source for some type of commercial subbing adhesive solution?  Thanks in advance for the help. 

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 355-6475 ext 1480
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu

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