[Histonet] (no subject)

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  Hello, everyone! I stained frozen and paraffin-embedded sections of hum=
an skeleton and kidney tissues using the Anti-=A6=C1-Dystroglycan antibod=
y, clone VIA4-1(catalog #: 05-298) from Upstate biotechnology company by =
immunohistochemistry. The method is: 3um sections, EDTA 8.0 heat retrieva=
l(pressure cooking) 10min for paraffin section=A3=ACantibody dilution is =
1:50,1:100 and 1:200(25 degree, 2 hours), and addition of EnVision antibo=
dy complex, and then DAB. But there was no any positive signals.=20
   I do not know what is the matter. Please help me. Now, I need to know =
whether the dilution or reaction time is suitable. Please give me some go=
od suggestions. I am eager to using the antibody smoothly. Thank you very=
 much.
Sincerely,
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Chunxia Zheng PhD
Research Institute of Nephrology, Jinling Hospital
305 East Zhongshan Road
Nanjing 210002, China
Phone: 86-25-80860218
Fax: 86-25-84801992

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=CE =20