[Histonet] Help with MDM2 Protein Immunostaining

[Young Kwun]

Dear Colleagues,

 

Does anyone in Histoland use MDM2 Protein staining for some soft tissue
sarcomas successfully?

I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's MDM2
(clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer.

However, my pathologist is not happy with the staining results, mainly
because of some non-specific stains. 

Any help would be appreciated. Thank you.

 

 

 

Young Kwun

Senior Hospital Scientist

Dept of Anatomical Pathology

Concord Hospital

Concord NSW 2139

Australia

Phone:61-2-9767 6075

Fax:61-2-9767 8427

Email:kwuny <@t> email.cs.nsw.gov.au