[Histonet] Cell Block preparation

[Tony Henwood]

I have used both agar amd plasma clot preparations for IPX.

If the sample has been fixed in solution prior to cell block preparation
then I have often had to resort to agar cell block preparations. Also if
the sample has a high concentration of proteases eg bile fluid, then
often the plasma clot won't form.

IPXs can often be problematical in agar cell blocks, probably due to the
deleterious effect of the hot agar. Plasma Cell Blocks are definitely
more consistent

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston,
Ronald
Sent: Wednesday, 30 May 2007 6:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block preparation


Would anyone care to comment on the pros and cons of preparing cell
blocks using the Plamsa/thrombin technique and the Agar method which is
more prominent in the European field?

 

I am particularly interested in the prevalence of background staining in
ICC. Does the use of plasma interfere with interpretation of the
staining results? I know there have been reports of extraneous tissue
being found in cell blocks coming from a commercially prepared clotting
agent.

 

Thanks

Ronnie

 

Ronnie Houston, MS, HT(ASCP)QIHC

Anatomic Pathology Manager

Columbus Children's Hospital

700 Children's Drive

Columbus, OH 43205

 

 



-----------------------------------------
Confidentiality Notice: This e-mail message, from Children's Hospital,
Columbus, Ohio, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. The recipient is responsible to maintain the
confidentiality of this information and to use the information only for
authorized purposes pursuant to Children's Hospital's confidentiality
policies. If you are not the intended recipient (or authorized to
receive information for the intended recipient), you are hereby notified
that any review, use, disclosure, distribution, copying, printing, or
action taken in reliance on the contents of this e-mail is strictly
prohibited. If you have received this communication in error, please
notify us immediately by reply e-mail and destroy all copies of the
original message. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************