[Histonet] Alfa & Beta Amylase

[anthony <@t> histotechexchange.com]

Dear Rene:

There are two types of Amylase, Alfa & Beta. Alfa will release Glucose and
maltose but Beta will only release maltose. You can get Diastase (meaning
it contains both Alfa & Beta)from Malt; try Fisher Scientific.

I would look into your procedure and ask your doctor if he is happy with
you saying you use Diastase when you are actually only using Alfa Amylase.

If you have the New Bancroft and Stevens it is on page 173.
Good luck and putting the subject in the header will get more people to
respond.

Yours truly,

Anthony Williams
Histotech Exchange LLC
19 Whitmore St.
Lexington, VA 24450
T 1 877 464 8911
F 1 540 301 0071
anthony <@t> Histotechexchange.com
www.histotechexchange.com


> concentration may be different and may affect the time you let it act, but
> usually diluted in the diastase buffer and incubated at 37ºC during 1
> hour, will be enough.
>   René J.
>
> Cheri Miller <cmiller <@t> physlab.com> wrote:
>   I need to know if I can use Amylase from Barley malt as opposed to
> Amylase
> from Pancreas for Diastase. Does it matter for diastase purposes??
>
> Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne
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