[Histonet] RE: IHC cytology specimens

[Anthony Reilly]

Hi

I would also like to add that although it may seem contrary to how we
treat paraffin sections air drying of your smears prior to fixation will
greatly assist in keeping the cells on the slide and has no adverse
affects on staining.

regards


Tony Reilly
Chief Scientist
Anatomical Pathology
QHPS-Prince Charles Hospital
Rode Rd Chermside Q 4032
Australia
Ph: 07 3139 4543
Fax: 07 3193 4546
tony_reilly <@t> health.qld.gov.au


>>> "Joanne Mauger" <MAUGER <@t> email.chop.edu> 05/25/07 10:37 pm >>>
Hi,

I agree with Chris. NBF actually seems to help tissue stay on slide
better. I will post fix a smear or cytospin in NBF for 15 mins. that
has
been previously fixed in alcohol. I do HIER as I would on FFPE.

I have found that some antibodies will not show expression unless this
post NBF fixation and retrieval has been done-eg. INI-1. I have also
found retrieval necessary on thin prep cytospins that had been only
fixed in sacamano,and 95% etoh.

Trial and error is the key.

Jo

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