[Histonet] DAPI staining methacrylate sections

[matt prideaux (RI)]

Hi everyone,

I'm trying to find a method to examine osteocyte density in areas of new
bone formation (between two calcein labels). The sections that we have
are 200 micron thick sections of mouse tibia. We have a confocal
microscope, which gives great images of the labels but the osteocytes
are not easily seen. I have heard that DAPI would be an ideal stain for
the osteocyte nuclei, but is it possible to stain methacrylate embedded
sections with DAPI and if so does anyone have a method for this?

Thanks,

Matt

Matt Prideaux
Bone Biology Group
Gene Function and Development
Roslin Institute
Roslin
Midlothian
EH25 9PS
Tel: +44 131 5274 244/254
Institute Fax: +44 131 440 0434

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