[Histonet] Tissue Tek Cryostat

[Gayle Callis]

A little trick to keep frost buildup at a minimum in an old Tissue Tek II 
cryostat.  After defrosting, we used to wipe down the flat metal surfaces 
of chamber with  ethylene glycol, or antifreeze, leaving a bit of this 
behind on the surface.  This kept frost from forming so fast, but do NOT 
get this on knife holder or blade or frozen blocks,  you do not want 
defrosted frozen sections.

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717


At 08:57 AM 5/24/2007, you wrote:
>Karen,
>I have an old Tissue Tek II as the "back-up" cryostat.  I monitor its 
>temperature everyday and found that the temperature I have it set at and 
>the actual temp varies considerably.  I had our bio-med guy look at it and 
>he said that there really isn't any thing he could do about it. So I watch 
>it closely.  I noticed that as frost starts to build up, the tempreature 
>goes up.  I know it's time to defrost it when the temp gets out of 
>range.  Once newly defrosted, it goes back to the colder end of the 
>range.  I have a seperate thermometer inside the chamber to get an acurate 
>reading for my QA log.  I do have a manual (copyright 1966!!) and it 
>doen't say anything about setting the temperature.
>Hope this helps,
>Anita
>------- Original message --------------
>From: "Heckford, Karen - SMMC-SF" <Karen.Heckford <@t> CHW.edu>
>
> > Good Morning Everyone, We have a old Tissue Tek 11 4553 cryostat. We
> > noticed after defrosting it that the temperature went way cold. We turned
> > the dial which moves the black hand and set it for -25 and noticed that 
> the
> > blue hand is way past -30. We cannot find the users guide to save our
> > lives. Probably lost a long time ago. Anyways can anyone tell me how to
> > reset this thing.
> > Cheers,
> >
> > Karen Heckford HT (ASCP) CE
> > Lead Histology Technician
> > Histology/Pathology Department
> > St. Mary's Medical Center
> > 450 Stanyan St.
> > San Francisco, Ca. 94117
> > 415-668-1000 ext. 6167
> > Fax: 415-750-8123
> > email: kheckfor <@t> chw.edu _______________________________________________
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610