[Histonet] RE: -20C acetone fixative

[C.M. van der Loos]

   Long  time ago they told me it is a milder fixative at -20C than at RT
   ???

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   -----Original Message-----
   From: histonet-bounces <@t> lists.utsouthwestern.edu
   [[1]mailto:histonet-bounces <@t> lists.utsouthwestern.edu]   On  Behalf  Of
   Charles,
   Roger
   Sent: Tuesday, May 22, 2007 12:29 PM
   To: histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] -20C acetone fixative
   Does any know or remember why acetone, when used as a fixative, is
   always used "ice cold" or in our case at -20C?
   Roger Charles
   Microbiologist
   Pennsylvania Veterinary Laboratory
   2305 N Cameron St
   Harrisburg, PA 17110
   717-787-8808

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