[Histonet] -20C acetone fixative

Tue May 22 14:25:02 CDT 2007

Because, if I'm not mistaken it blows up very easily.

Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles,
Roger
Sent: Tuesday, May 22, 2007 12:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] -20C acetone fixative

Does any know or remember why acetone, when used as a fixative, is
always used "ice cold" or in our case at -20C? 

Roger Charles

Microbiologist

Pennsylvania Veterinary Laboratory

2305 N Cameron St

Harrisburg, PA 17110

717-787-8808

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