[Histonet] Microtome calibration?

[Bernice Frederick]

We actually had ours "calibrated by the guys who did our PM. They can do
it- just ask
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
AGrobe2555 <@t> aol.com
Sent: Tuesday, May 22, 2007 9:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtome calibration?

Good morning,
A question was raised as to "how do you know the sections are  the 
appropriate thickness?".  Does one trust the settings on the  microtome
(Leitz 1512)?  
Is it an experienced eye that knows when they are  "right"?  Or is there
some 
instrument that will do this measurment?   Is there some way to
calibrate the 
microtome to ensure that the sections  actually are Xum?
 
Thanks,
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital






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