[Histonet] Microtome calibration?

[AGrobe2555 <@t> aol.com]

Good morning,
A question was raised as to "how do you know the sections are  the 
appropriate thickness?".  Does one trust the settings on the  microtome (Leitz 1512)?  
Is it an experienced eye that knows when they are  "right"?  Or is there some 
instrument that will do this measurment?   Is there some way to calibrate the 
microtome to ensure that the sections  actually are Xum?
 
Thanks,
Albert  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital






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